Production and identification of a novel compound, 7,10-dihydroxy-8(<Emphasis Type="Italic">E</Emphasis>)-hexadecenoic acid from palmitoleic acid by <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> PR3 |
| |
Authors: | Jae-Han Bae Deuk-Soo Kim Min-Jung Suh Sei-Ryang Oh In-Jung Lee Sun-Chul Kang Ching T Hou Hak-Ryul Kim |
| |
Affiliation: | (1) Department of Animal Science and Biotechnology, Kyungpook National University, Daegu, 702-701, South Korea;(2) Natural Medicine Research Center, KRIBB, Daejeon, South Korea;(3) Division of Plant Biosciences, Kyungpook National University, Daegu, South Korea;(4) Department of Biotechnology, College of Engineering, Daegu University, Gyungsan City, South Korea;(5) Microbial Genomic and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, Peoria, IL 61604, USA |
| |
Abstract: | Hydroxy fatty acids are considered as important value-added product for industrial application because of their special properties
such as higher viscosity and reactivity. Microbial production of the hydroxy fatty acids from various fatty acid substrates
have been actively studied using several microorganisms. The new bacterial isolate Pseudomonas aeruginosa (PR3) had been reported to produce mono-, di-, and tri-hydroxy fatty acids from different unsaturated fatty acids. Of those,
7,10-dihydroxy-8(E)-octadecenoic acid (DOD) and 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD) were produced from oleic acid and ricinoleic acid, respectively. Based on the postulated common
metabolic pathway involved in DOD and TOD formation by PR3, it was assumed that palmitoleic acid containing a singular 9-cis double bond, common structural property sharing with oleic acid and ricinoleic acid, could be utilized by PR3 to produce
hydroxy fatty acid. In this study, we tried to use palmitoleic acid as substrate for production of hydroxy fatty acid by PR3
and firstly confirmed that PR3 could produce 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) with 23% yield from palmitoleic acid. DHD production was peaked at 72 h after the substrate was
added to the 24-h-culture. |
| |
Keywords: | Pseudomonas aeruginosa Palmitoleic acid Dihydroxy fatty acid Bioconversion |
本文献已被 PubMed SpringerLink 等数据库收录! |
|