黄芪有效部位不同提取分离方法的比较

目的:建立快速测定中药样品中非法添加的化学药物的液相色谱一质谱联用分析方法.方法:采用串联质谱联用技术的多反应监测模式,对分属于降糖、降压、壮阳、减肥等类别的36种中药,进行其中可能含有的格列本脲、西布曲明等10种化学药物的定性、定量分析.结果:在10 min色谱未完全分离的情况下,在36个样品中准确地检出了4个降糖类样品(合格列本脲)、1个壮阳类样品(含西地那非)、2个减肥类样品(含西布曲明).结论:多反应监测(MRM)模式确保了定性分析结果的专属性和定量分析的灵敏度,建立的方法可用于多种不同样品中化学药物的检验.

Comparison of different extraction and separation methods on the effective sections of Radix Astragali

Objective:To select optimal diameter of the powder of Radix Astragali and extraction method by four methods of extracting astragalus polysaccharide and total astragalus saponins in three Radix Astragali powders of different diameter.Methods:The water-alcohol co-distillation, cellulose degradation technology, calcarea solution technology, ultrasonic waves technology were used to three Radix Astragali powders of different diameter respectively.The astragalus polysaccharide and total astragalus saponins were separated with combined techniques hollow fiber membrane and D21 macro absorbing resin. According to the contents of astragalus polysaccharide and total astragalus saponins,the extraction methods were compared.Results:The most effective way of extracting astragalus polysaccharide was the ultrasonic waves technology with the yield rate of 1.73%, while the most effective method of distilling total astragalus saponins was ultrasonic waves technology on prepared slice of herbal drug, with the yield of 1.82.Conclusion:Ultrasonic waves technology was a much better way of extracting both astragalus polysaccharide and total astragalus saponins. The shorter the diameter of Radix Astragali powder, the higher the yield of extracting astragalus polysaccharide. The yield of extracting total astragalus saponins turned out contray.