| 铜绿假单胞菌临床分离株16S rRNA甲基化酶基因研究 |
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| 引用本文： | 钱雅芬,倪舒芳,孙婷婷,滕艳文,毛翠,周洪昌.铜绿假单胞菌临床分离株16S rRNA甲基化酶基因研究[J].抗感染药学,2011,8(2):92-94. |
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| 作者姓名： | 钱雅芬 倪舒芳 孙婷婷 滕艳文 毛翠 周洪昌 |
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| 作者单位： | 湖州师范学院医学院,创新实验室,浙江,湖州,313000 |
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| 基金项目： | 浙江省科技厅新苗人才计划 |
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| 摘 要： | 目的:了解临床分离铜绿假单胞菌氨基糖苷类药物耐药性与16SrRNA甲基化酶基因的关系,探讨多药耐药机制。方法:收集20株铜绿假单胞菌临床标本,采用K-B法检测20株铜绿假单胞菌对卡那霉素、阿米卡星、妥布霉素、庆大霉素、奈替米星的敏感性;采用PCR法检测5种(armA,rmtA,rmtB,rmtC,rmtD)16SrRNA甲基化酶基因。结果:10株铜绿假单胞菌对上述5种氨基糖苷类药物全部耐药,1株对阿米卡星敏感,对另外4种药物表现为耐药,6株对1~2种药物表现为耐药,其余3株对5种药物全部敏感。基因检测显示rmtB和armA阳性率为15%,未检测到rmtA,rmtC,rmtD基因。结论:铜绿假单胞菌对氨基糖苷类药物耐药情况严重,并在其中检出16SrRNA甲基化酶基因,应引起重视。
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| 关 键 词： | 铜绿假单胞菌 16SrRNA 甲基化酶基因 氨基糖苷类 |
Study on 16S rRNA Methylase Genes in Pseudomonas Aeruginosa |
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| QIAN Ya-fen,NI Shu-fang,SUN Ting-ting,TENG Yan-wen,MAO Cui,ZHOU Hong-chang.Study on 16S rRNA Methylase Genes in Pseudomonas Aeruginosa[J].Anti-infection Pharmacy,2011,8(2):92-94. |
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| Authors: | QIAN Ya-fen NI Shu-fang SUN Ting-ting TENG Yan-wen MAO Cui ZHOU Hong-chang |
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| Affiliation: | QIAN Ya-fen*2,NI Shu-fang,SUN Ting-ting,TENG Yan-wen,MAO Cui,ZHOU Hong-chang*3 Medical school of Huzhou Teachers College,Huzhou Zhejiang 313000,China |
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| Abstract: | Objective:To investigate the antibiotic resistance and 16S rRNA methylase gene of the multidrug-resistant Pseudomonas aeruginosa and to discuss the mechanism.Methods:20 samples of Pseudomonas aeruginosa isolates were collected from hospital.K-B method was used to determine the sensitivity to 5 antibacterials including kanamycin,amikacin,tobramycin,gentamicin and netilmicin,and the 16S rRNA methylase genes were amplified by polymerase chain reaction(PCR).Results:10 strains of Pseudomonas aeruginosa were resi... |
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| Keywords: | 16S rRNA |
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