天麻素对缺血再灌注神经细胞膜的保护作用

用新生Wistar大鼠大脑皮层进行神经细胞培养 ,将培养 8d的神经细胞置于模拟“缺血”状态下 ,5h后神经细胞发生肿胀 ,胞内乳酸脱氢酶 (LDH)漏出增加 ,膜流动性下降、脂质过氧化(LPO)含量增加 ;将经过“缺血”损伤神经细胞置 1 8h模拟“再灌注” ,“再灌注”后细胞数目明显减少 ,LDH的漏出、膜流动性下降和LPO增加继续加重 ,说明细胞损伤进一步加重。经过天麻素孵育后的神经细胞“缺血”或“再灌注”后LDH的漏出及LPO含量明显低于损伤组 ,而膜流动性则明显高于损伤组。表明 :天麻素对体外培养神经细胞的缺血再灌注损伤有保护作用

Protective Effects of Gastrodine on Neuronal Membrane Shown in the Neuronal Ischemia and Reperfusion Experiment

Cerebral cortex of the newborn rat was used for the culture of neurons. The neurons from 8 days' culture were put into an environment of simulated ischemia, and after 5 hours, they became swollen with increased LDH efflux, decreased biomembrane fluidity and increased lipid peroxide content. The neurons undergone simulated ischemia were put into an environment of simulated reperfusion, and after 18 hours, their number was decreased sharply with markedly increased LDH efflux and lipid peroxide content and markedly decreased biomembrane fluidity. However, the neurons incubated with gastrodine were less damaged when they underwent the same two simulated environments, which suggests that gastrodine can resist the injury caused by ischemia and reperfusion.