| 反相高效液相色谱法测定灵芝子实体中灵芝酸A的含量 |
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| 引用本文： | 杨娟娟,李晔,魏巧容,吴长辉.反相高效液相色谱法测定灵芝子实体中灵芝酸A的含量[J].福建医药杂志,2011,33(3):56-58. |
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| 作者姓名： | 杨娟娟 李晔 魏巧容 吴长辉 |
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| 作者单位： | 福建仙芝楼生物科技有限公司,福州,350002 |
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| 摘 要： | 目的 建立测定灵芝子实体中灵芝酸A含量的反相高效液相色谱法(RP-HPLC)测定方法.方法 采用Promosil-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-体积分数0.03％甲酸水溶液为流动相梯度洗脱,流速为0.8 ml/min,检测波长254 nm.结果 灵芝酸A的质量浓度在1.1～11μg范围内与...
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| 关 键 词： | 灵芝子实体 灵芝酸A 反相高效液相色谱法 含量测定 |
RP-HPLC method of determining the content of Ganoderma lucidum acid A in its fruiting body |
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| YANG Juan-juan,LI Ye,WEI Qiao-rong,WU Chang-hui.RP-HPLC method of determining the content of Ganoderma lucidum acid A in its fruiting body[J].Fujian Medical Journal,2011,33(3):56-58. |
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| Authors: | YANG Juan-juan LI Ye WEI Qiao-rong WU Chang-hui |
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| Affiliation: | .Fujian Xianzhilou Biological Science&Technology Co.,Ltd.,Fuzhou 350002,China |
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| Abstract: | Objective To establish RP-HPLC method of determining the content of Ganoderma lucidum acid A in its fruiting body.Methods We adopted Promosil-C18(250 mm×4.6 mm,5 μm) column and used acetonitrile-0.03% volume fraction of formic acid as the mobile phase gradient elution with flow rate 0.8 ml/minand detection wavelength 254 nm.Results The concentration range of Ganoderma lucidum acid A was in 1.1-11 μg and the peak area showed good linearity,r=0.999,Y= 1.121X-0.112,RSD= 1.61%(n=6).Conclusion The method is simple,rapid,accurate,repeated,and can be used to control the quality of Ganoderma lucidum fruiting bodies. |
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| Keywords: | Ganoderma lucidum fruiting body Ganoderma lucidum acid A RP-HPLC determination of content |
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