CD55,CD59抗原表达在PNH合并全血细胞减少症诊断中的应用

目的 探讨红细胞和粒细胞膜CD55与CD59抗原表达在阵发性睡眠性血红蛋白尿症(PNH)合并全血细胞减少症(PCP)诊断中的应用.方法采用流式细胞术结合直接免疫荧光法,检测PCP患者病因可疑为PNH的的外周血红细胞和粒细胞膜 CD55与CD59抗原的表达.结果 125例中,PNH32例,非PNH 93例(包括再生障碍性...

Application of CD55 and CD59 Antigen Expressions in the Diagnosis of PNH Complicated with Pancytopenia

Objective To discuss the application of CD55 and CD59 antigen expressions of the erythrocyte and granulocyte cell membranes in the diagnosis of paroxysmal nocturnal hemoglobinuria(PNH) complicated with pancytopenia.Methods Flow cytometry and direct immuofluorescence staining were used to detect the expressions of CD55 and CD59 antigens in the cell membranes of peripheral erythrocytes and granulocytes in pancytopenia with susceptible etiology of PNH.Results Thirty-two of the 125 cases were PNH,and 93 were non-PNH including 80 cases of aplastic anemia and 13 cases of pancytopenia with unknown etiology.The negative expressions of the CD55 and CD59 antigens of the peripheral erythrocytes have 93.75% sensitivity and 82.80% specificity in the diagnosis of PNH complicated with pancytopenia.There is 100% sensitivity and 81.72% specificity for the diagnosis based on the negative expressions of the CD55 and CD59 antigens of the granulocyte cell membranes.It is known from the subsequent follow-up of the patents that 13 of the non-PNH cases were transformed into PNH.The negative expression rates of these patient cases are 84.62% and 92.31% respectively for the CD55 and CD59 antigens in the erythrocyte cell membrane,and 92.31% and 100% respectively in the granulocyte cell membrane.Conclusion The simultaneous detection of patients’ CD55 and CD59 antigens in the cell membranes of erythrocytes and granulocytes by flow cytometry is significantly valuable in the diagnosis and differential diagnosis of PNH complicated with pancytopenia.