胃癌患者唾液蛋白质组学分析

目的 应用差异蛋白质组学方法筛选唾液中与胃癌相关的蛋白质分子.方法 采用双向凝胶电泳(2-DE)分离胃癌患者和正常人群唾液总蛋白,从中选取差异表达蛋白质点,通过基质辅助激光解吸电离飞行时间串联质谱(MALD I-TOF-TOF-MS)鉴定差异表达的蛋白质;Western blot 验证蛋白质组学分析的结果.结果 通过两组人群唾液的双向凝胶电泳图谱分析发现15个差异蛋白质斑点,质谱鉴定出9种蛋白质,在胃癌患者唾液中高表达的有3个,其余6个在胃癌患者唾液中低表达.Western blot对表达增高的蛋白鸟嘌呤核苷酸解离抑制因子2(RhoGDI2)进行检测,结果与蛋白质组学分析结果一致.结论 蛋白质组学研究是筛选胃癌患者唾液中特异性标记物的有效手段.

Saliva protein comparative proteomics in patients with gastric cancer

Objective To screen the saliva proteins associated with the gastric carcinoma using a comparative proteomic approach.Methods 2-DE wag applied to separate the whole saliva proteins in gas-tric cancer and paired normal group.The proteins with statistically significant differential expression be-tween the gastric carcinoma and normal groups were identified by matrix-assisted laser desorption/ionization tandem time-of-flisat mass spectrometry(MALD I-TOF-TOF-MS).Western blotting Was performed to ex-amine the differential expression of the candidate proteins.Results Fifteen protein spots were found as differentially expressed proteins,in which 9 proteins were identified successfully.Among the identified pro-teins,three were over-expressed and six under-expressed in whole saliva of gastric cancer patients as com-pared with normal group.GDP-dissociation inhibitor-2 wag found to be over-expressed in saliva of gastric cancer patients in comparison with the normal group,as confirmed by Western blotting.Conclusion Comparative proteomics analysis provides effective approach to screen the special biological markers in saliva for gastric cancer patients.