| 应用酶联免疫吸附试验快速定量检测狂犬病疫苗免疫人体后的抗体水平 |
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| 引用本文： | 刘瑜瑄,黄浩,李淑云,星海,唐巧英.应用酶联免疫吸附试验快速定量检测狂犬病疫苗免疫人体后的抗体水平[J].中国计划免疫,2000,6(3). |
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| 作者姓名： | 刘瑜瑄 黄浩 李淑云 星海 唐巧英 |
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| 作者单位： | 北京生物制品研究所!北京100024 |
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| 摘 要： | 用酶联免疫吸附试验 (ELISA)间接法检测狂犬病疫苗免疫人体后的抗体含量 ,共检测 93份人血清 ,结果表明 :敏感度为0 5 1IU/0 1ml,特异性强 ,重复性好 ,变异系数为 9 82 %。与小鼠中和试验 (MNT)比较 ,二者具有平行关系 ,敏感性优于MNT ,符合率为 98 9%。本方法改变了以往ELISA法有滴度无国际单位的情形 ,对快速定量检测狂犬病疫苗免疫人体后的抗体水平、流行病学调查等项工作具有一定的应用价值
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| 关 键 词： | 狂犬病疫苗 抗体 酶联免疫吸附试验 小鼠中和试验 |
Papid Quantitative Assay of Rabies Post-vaccination Antibody by ELISA |
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| LIU Yu-xuan,HUANG Hao,LI Shu-yun,et al..Papid Quantitative Assay of Rabies Post-vaccination Antibody by ELISA[J].Chinese Journal of Vaccines and Immunization,2000,6(3). |
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| Authors: | LIU Yu-xuan HUANG Hao LI Shu-yun |
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| Affiliation: | LIU Yu-xuan,HUANG Hao,LI Shu-yun,et al. Beijing Institute of Biological Products,Beijing 100024,China. |
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| Abstract: | An indirect ELISA for rabies post-vaccination antibody determination has been developed. A total of 93 human serum samples were tested, the results showed strong specificity and good reproducibility. The sensitivity could reach 0.51 IU/0.1ml and the coefficient of variation was 9.82%. The sera were also tested by mice neutralization test(MNT), the results were parallel with ELISA method and their coincidence rate was 98.9%. The sensitivity of indirect ELISA assay was higher than MNT. The results of ELISA thus can be reported as international units per 0.1 milliliter (IU/0.1ml). It can be recommended as a rapid, sensitive and reliable technique for quantitative assay of rabies post-vaccination antibody. |
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| Keywords: | Rabies vaccine Post-vaccination antibody ELISA MNT |
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