| 浓香型白酒醅中高产酒精酵母菌的定向筛选及分子鉴定 |
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| 引用本文： | 曾娟,施思,吴正云,张文学,胡峰,赵鸿飞,李波.浓香型白酒醅中高产酒精酵母菌的定向筛选及分子鉴定[J].中国酿造,2011(2). |
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| 作者姓名： | 曾娟 施思 吴正云 张文学 胡峰 赵鸿飞 李波 |
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| 作者单位： | 1. 四川大学轻纺与食品学院,四川,成都,610065
2. 贵州茅台酒厂(集团)习酒有限责任公司,贵州,习水,564622 |
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| 基金项目： | 贵州省科技厅重大专项项目 |
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| 摘 要： | 为了获得适用于酿酒生产的高产酒精酵母菌株,从浓香型白酒醅样品中,经不同浓度乙醇和pH条件下的定向富集和耐受性筛选以及发酵实验,获得3株能在酒精度13%vol、pH3.50的条件下稳定生长且产酒能力较强的酵母菌)XP-3、XP-7和XP-8,其中XP-3的产酒力达87.04%,粮食出酒率可达44.71%,高于一般酿酒酵母.18S rDNA序列分析结果表明,3株酵母菌均与酿酒酵母(Saccharomyces cerevisiae)具有极高的相似性.
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| 关 键 词： | 白酒酒醅 高产酒精酵母菌 定向筛选 分子鉴定 |
Directional selection and molecular analysis of yeast strains with high alcohol yield in fermented grains of strong-flavor liquor |
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| ZENG Juan,SHI Si,WU Zhengyun,ZHANG Wenxue,HU Feng,ZHAO Hongfei,LI Bo.Directional selection and molecular analysis of yeast strains with high alcohol yield in fermented grains of strong-flavor liquor[J].China Brewing,2011(2). |
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| Authors: | ZENG Juan SHI Si WU Zhengyun ZHANG Wenxue HU Feng ZHAO Hongfei LI Bo |
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| Affiliation: | ZENG Juan1,SHI Si1,WU Zhengyun1,ZHANG Wenxue1,HU Feng2,ZHAO Hongfei2,LI Bo2(1.College of Textile & Food Science,Sichuan University,Chengdu 610065,China,2.Xijiu Co.Ltd.,of Moutai Group,Xishui 564622,China) |
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| Abstract: | In order to obtain yeast strains with high alcohol yield in brewing,three yeasts(XP-3,XP-7 and XP-8) were obtained from fermented grains of strong-flavor liquor through directional selection and tolerant experiment.The three yeast strains grew well under the 13% vol ethanol and pH 3.5.The highest alcohol yield-rate(87.04%) and the alcohol yield-rate of grain(44.71%) were obtained in XP-3.It was found that the yeast strains have the high similarity with Saccharomyces cerevisiae through the sequences analysis... |
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| Keywords: | fermented grains Chinese liquor high alcohol-yield yeast directional selection molecular analysis |
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