| 柱前衍生-高效液相色谱法测定暗纹东方鲀肉中牛磺酸含量 |
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| 引用本文： | 王丽雅,陶宁萍,张恒劼.柱前衍生-高效液相色谱法测定暗纹东方鲀肉中牛磺酸含量[J].食品与发酵工业,2012,38(10):159-164. |
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| 作者姓名： | 王丽雅 陶宁萍 张恒劼 |
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| 作者单位： | 上海海洋大学食品学院,上海,201306 |
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| 基金项目： | 上海市教委“食品质量与安全”重点学科建设项目，上海市科委工程中心建设，镇江凯普商贸长江生态养殖中心“养殖河豚营养评价及风味比较研究” |
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| 摘 要： | 运用柱前衍生-高效液相色谱法测定暗纹东方鲀肉中牛磺酸的含量,以邻苯二甲醛为衍生剂,样品液与衍生剂进行衍生反应的体积比为1∶1,反应时间为1 min,甲醇-乙腈-水(体积比10∶20∶70)为流动相,采用XbridgeTMC18色谱柱,波长为330 nm,流速1 mL/min,进样量20μL的条件下检测样品中的牛磺酸。结果表明:牛磺酸的线性范围为5～30μg/mL,相关系数R2为0.999 1,回收率范围95.35%～98.19%,变异系数(RSD)=2.03%～3.71%(n=3),样品6次重复测定的RSD=0.51%,最小检出限1.14μg/mL。
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| 关 键 词： | 牛磺酸 高效液相色谱法 柱前衍生 邻苯二甲醛 暗纹东方鲀 |
Determination of Taurine in Takifugu obscurus by Pre-column Derivatization High Performance Liquid Chromatographic |
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| Wang Li-ya,Tao Ning-ping,Zhang Heng-.Determination of Taurine in Takifugu obscurus by Pre-column Derivatization High Performance Liquid Chromatographic[J].Food and Fermentation Industries,2012,38(10):159-164. |
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| Authors: | Wang Li-ya Tao Ning-ping Zhang Heng- |
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| Affiliation: | jie(College of Food Science & Technology,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | A pre-column derivatization high-performance liquid chromatography(HPLC) method for the determination of taurine in Takifugu obscurus was developed.Taurine was derivatized with Ophthalaldehyde(OPA).The derivatives were separated on a reversed-phase XbridgeTM C18 column by a mobile phase by(A) methanol,(B) acetonitrile and(C) water(10/20/70).Sample size was about 20 μL,flow rate was about 1 mL/min and detection was done at 330 nm by ultraviolet detector.Results showed that the linear ranger of taurine was 5~30μg/mL,correlation coefficient R2=0.9991,the recoveries were 95.35%~98.19%,RSD=2.03~3.71%(n=3).The precision RSD = 0.51%(n=6) and the detection limit was1.14μg/mL.The method is simple,quick,specific,sensitive,repetitively and accurate. |
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| Keywords: | taurine HPLC pre-column derivatization OPA Takifugu obscurus |
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