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| H.11648麻离体培养与植株再生的研究 | |
| 引用本文： | 张世清,易克贤,郑金龙,陈河龙,刘巧莲,高建明.H.11648麻离体培养与植株再生的研究[J].江西农业学报,2012,24(4):17-19. |
| 作者姓名： | 张世清 易克贤 郑金龙 陈河龙 刘巧莲 高建明 |
| 作者单位： | 1. 中国热带农业科学院 热带生物技术研究所、农业部热带作物生物学与遗传资源利用重点实验室,海南 海口 571101;海南大学 农学院,海南 海口 570208 2. 中国热带农业科学院 环境与植物保护研究所,海南 儋州,571737 3. 中国热带农业科学院 热带生物技术研究所、农业部热带作物生物学与遗传资源利用重点实验室,海南 海口,571101 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助，国家麻类现代产业技术体系建设专项 |
| 摘 要： | 以H.11648麻组培苗叶片为外植体,研究了不同生长调节剂对诱导愈伤、不定芽的分化、增殖及生根的影响。结果表明:在MS+0.5 mg/L 2,4-D+4.0 mg/L 6-BA+0.5 mg/L NAA+3%蔗糖+0.23%植物凝胶(pH值5.8)培养基上诱导出愈伤组织为佳;在MS+3.0 mg/L 6-BA+0.3 mg/L NAA培养基中,愈伤组织不定芽分化率最高;将小苗移栽至基质配比为土壤∶椰糠=2∶1的育苗盆中,成活率达96%。 |
| 关 键 词： | 剑麻 叶片 植株再生 愈伤 |
Study on Isolated Culture and Plant Regeneration of Agave sisalana H.11648 |
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| ZHANG Shi-qing , YI Ke-xian , ZHENG Jin-long , CHEN He-long , LIU Qiao-lian , GAO Jian-ming.Study on Isolated Culture and Plant Regeneration of Agave sisalana H.11648[J].Acta Agriculturae Jiangxi,2012,24(4):17-19. | |
| Authors: | ZHANG Shi-qing YI Ke-xian ZHENG Jin-long CHEN He-long LIU Qiao-lian GAO Jian-ming |
| Affiliation: | 1(1.Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture,Haikou 571101,China;2.Institute of Environment and Plant Protection,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,China;3.College of Agronomy,Hainan University,Haikou 570208,China) |
| Abstract: | Using the leaves of test-tube seedlings of Agave sisalana H.11648 as the explants,the effects of different growth regulating substances on the callus inducement,differentiation,proliferation and rooting of adventitious buds were researched.The results indicated that the highest callus inducement frequency was obtained on the medium MS+0.5 mg/L 2,4-D+4.0 mg/L 6-BA+0.5 mg/L NAA+3% cane sugar+0.23% plant gelatum(pH 5.8),and the optimum medium for the differentiation of the adventitious buds from callus was MS+3.0 mg/L 6-BA+0.3 mg/L NAA.The survival rate of the test-tube seedlings was 96% after they were transplanted into the nursery pot with the substrate of soil+coconut bran(2∶1). |
| Keywords: | Agave sisalana Leaf Plant regeneration Callus |
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