| STAT3RNAi联合γ射线照射对U251细胞增殖的影响 |
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| 引用本文： | 高玲,李峰生,董波,张军权,饶亚岚,王治东,丛悦,毛秉智,陈肖华.STAT3RNAi联合γ射线照射对U251细胞增殖的影响[J].中华放射医学与防护杂志,2008,28(1):17-20. |
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| 作者姓名： | 高玲 李峰生 董波 张军权 饶亚岚 王治东 丛悦 毛秉智 陈肖华 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所,北京,100850 |
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| 摘 要： | 目的 构建特异性抑制信号转导与转录激活因子3 (STAT3)的小干扰RNA(siRNA)表达载体并检测联合 60Co γ射线照射对U251细胞STAT3表达及细胞增殖的抑制作用。方法 设计、合成STAT3特异性的19 bp短链寡核苷酸,经退火形成双链DNA片段,克隆到pSilence2.1-U6-H1载体中,构建STAT3特异siRNA的表达载体, HindⅢ和BamHⅠ双酶切及测序鉴定重组体,并转染U251细胞,免疫印迹法(Western blot)检测STAT3 mRNA和蛋白的表达水平,四甲基偶氮唑蓝(MTT)检测细胞增殖活性,克隆形成率及MTT实验确定放疗剂量。结果 经双酶切与测序鉴定成功构建pSilence2.1-STAT3表达载体,转染星形胶质瘤细胞株U251细胞可显著抑制细胞中STAT3 蛋白的表达水平;确定2 Gy为放疗剂量。转染pSilence2.1-STAT3,细胞增殖活性较未转染U251细胞显著降低(P<0.05),联合 60Co γ射线照射U251细胞增殖活性单独转染进一步显著降低(P<0.05)。结论 运用pSilence2.1-U6-H1载体构建的pSilence2.1-STAT3表达载体可有效抑制STAT3的表达及星形胶质瘤细胞的增殖; 联合2 Gy 60Co γ射线照射可增加抑制效果。
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| 关 键 词： | 小干扰RNA 转录激活因子3 U251细胞 |
| 收稿时间： | 3/7/2006 12:00:00 AM |
Effect of combination of STAT3 RNAi and 60Co γ-irradiation on U251 cell proliferation |
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| GAO Ling,LI Feng-sheng,DONG Bo.Effect of combination of STAT3 RNAi and 60Co γ-irradiation on U251 cell proliferation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):17-20. |
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| Authors: | GAO Ling LI Feng-sheng DONG Bo |
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| Affiliation: | Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Beijing Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | |
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| Keywords: | Small interfering RNA STAT3 U251 cell line |
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