中药“神经再生素”促神经生长过程中基因差异性表达的初步研究

为了探索中药“神经再生素”促神经生长过程中基因水平的变化。本实验采用 dd-PCR方法 ,从体外培养背根神经节细胞加药组和不加药组中获得两者的差异表达片段 ,并经反杂交筛选、克隆测序、DNA序列检索分析、Northern验证。结果表明 ,差异显示共获得 8个差异条带 ,一个为下调基因 ,其余为上调基因 ,其中 2个 c DNA序列与 RRAJ5 16 1基因 (增殖相关基因 )、AF196 3 15基因 (锌指样蛋白 DDP2 ) 10 0 %同源 ,2个 c DNA序列与 AK0 0 175 7基因、STA5 SRR基因 (t RNA合成酶 )部分同源。结论是中药“神经再生素”在促神经生长过程中 ,对神经元基因的选择性表达起着重要的调控作用。

THE PRELIMINARY STUDY OF GENE DIFFERENTIAL EXPRESSION DURING NERVE GROWTH WITH THE EFFECT OF "NERVE REGENERATION FACTOR"

To explore the molecular mechanism of the effect of "Nerve Regeneration Factor" upon nerve growth. Differential display PCR(dd PCR) was used to detect the different gene expression level of two groups of cultured cells from dorsal root ganglia(DRG), one treated with "Nerve Regeneration Factor" and the other not. The differential cDNA fragments were screened primarily by reverse hybridization, subsequently cloned and sequenced, then further confirmed by Northern blot. Eight differential fragments were obtained. Comparing these sequence to data from GenBank showed that one was completely homologous to RRAJ5161(proliferating correlative gene), one to AF196315(small zinc finger like protein DDP2), and two other fragments showed partial homology to AK001757 and STA5SRR(lysyl tRNA synthetase) respectively. This study indicates "Nerve Regeneration Factor" plays an important role in regulating gene expression during stimulating nerve growth.