| 人前列腺干细胞的分离培养 |
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| 引用本文： | 王共先,傅斌,汪泱,曹润福,黄学明,崔苏萍,刘伟鹏,张中华,胡红林,胡银英.人前列腺干细胞的分离培养[J].江西医学院学报,2004,44(1):1-3,F002. |
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| 作者姓名： | 王共先 傅斌 汪泱 曹润福 黄学明 崔苏萍 刘伟鹏 张中华 胡红林 胡银英 |
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| 作者单位： | [1]江西医学院第一附属医院泌尿外科，江西医学院泌尿外科研究所，江西南昌330006 [2]江西省医学科学研究所，江西南昌330006 |
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| 摘 要： | 目的 探讨人前列腺干细胞的分离方法并初步鉴定其特性。方法 以器官捐献者的正常前列腺为研究对象，利用免疫磁珠细胞分离和胶原快速粘附方法，从前列腺基底细胞中分离前列腺干细胞．研究其克隆形成和分化特性。结果 从正常人前列腺内分离得到的细胞．能快速粘附胶原，克隆形成率明显高于未粘附的前列腺基底细胞，而且形成的克隆维持时间较长。结论 人前列腺基底细胞中存在极少量的具有高度增殖能力的一群细胞．它们具有干细胞的特征．但有待于进一步研究鉴定。
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| 关 键 词： | 前列腺干细胞 细胞分离 免疫磁珠 细胞外基质 |
| 文章编号： | 1000-2294(2004)01-0001-03 |
Isolation and Cultivation of Normal Human Prostate Stem Cells |
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| WANG Gong-xian ,FU Bin ,WANG Yang ,CAO Run-fu ,HUANG Xue-ming ,CUI Su-ping ,LIU Wei-peng ,ZHANG Zhong-hua ,HU Hong-lin ,HU Yin-ying.Isolation and Cultivation of Normal Human Prostate Stem Cells[J].Acta Academiae Medicinae Jiangxi,2004,44(1):1-3,F002. |
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| Authors: | WANG Gong-xian FU Bin WANG Yang CAO Run-fu HUANG Xue-ming CUI Su-ping LIU Wei-peng ZHANG Zhong-hua HU Hong-lin HU Yin-ying |
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| Affiliation: | WANG Gong-xian 1,FU Bin 1,WANG Yang 1,CAO Run-fu 1,HUANG Xue-ming 1,CUI Su-ping 1,LIU Wei-peng 1,ZHANG Zhong-hua 1,HU Hong-lin 1,HU Yin-ying 2 |
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| Abstract: | Objective To explore the methods of isolating the normal human protate stem cells and to identify its characteristics. Methods Using immunologic magnetic beads and the characteristics of stem cells fast adhering to collagen, clonal analysis of normal human prostate putative stem cells from basal cells were undertaken to identify stem cells.Results A small group of cells isolated from prostatic basal cells were characterized by fast adhesion to collagen I and high clone forming efficiency(CFE),which was much more high than that of the homogenetic prostatic basal cells.Conclusion On the basis of their high proliferative characteristics,the group of cells may be prostate stem cells or their progeny. |
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| Keywords: | human prostate stem cells cell isolation immunologic magnetic microbeads extracellular matrix |
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