Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas |
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| Affiliation: | 1. Fonterra Co-operative Group Ltd, P.O. Box 7, Waitoa, New Zealand;2. R J Hill Laboratories Ltd, Clyde St, Hamilton, New Zealand;1. Faculdade de Enfermagem, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil;2. Secretaria Municipal de Saúde, Catalão, GO, Brazil;3. Instituto de Biotecnologia, Universidade Federal de Goiás (UFG), Catalão, GO, Brazil;1. Virology Section, Laboratory of Immunopathology Keizo Asami, Federal University of Pernambuco, Recife, Pernambuco, Brazil;2. Department of Virology and Experimental Therapy, Aggeu Magalhães Institute, Foundation Oswaldo Cruz, Pernambuco, Brazil;3. Virology Section, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil;4. Department of Physiology and Pharmacology, Center of Biosciences, Federal University of Pernambuco, Recife, Pernambuco, Brazil |
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| Abstract: | A method for the simultaneous determination in milk of the 5′-mononucleotides adenosine 5′-monophosphate, cytidine 5′-monophosphate, guanosine 5′-monophosphate, inosine 5′-monophosphate and uridine 5′-monophosphate and their corresponding nucleosides is described. Following deproteinisation, the sample extract was analysed by reversed-phase liquid chromatography, whereby chromatographic separation was achieved using a polymer grafted silica C18 column, gradient elution with a simple binary mobile phase and UV detection by photodiode array. Performance parameters included recoveries of 95.5–105.2% and precision evaluated as 3.42–6.38% relative standard deviation. The described technique has been applied to the analysis of bovine and human milk, a range of commercial bovine milk-based infant and follow-on formulas, a seasonal study of skim milk powders and an assessment of alkaline phosphatase influence on nucleotide retention. |
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