方格星虫多糖对5.0Gy ^60Coγ辐射损伤小鼠的保护作用

目的观察方格星虫多糖对亚致死剂量60Coγ射线照射小鼠的保护作用。方法将60只雄性C57BL/6J小鼠随机分为正常对照组、模型组、尼尔雌醇组及低（100 mg/kg·d）、中（200 mg/kg·d）、高（400 mg/kg·d）三个剂量方格星虫多糖组,每天一次灌胃给药,尼尔雌醇组照射前一天给予尼尔雌醇3.3 mg/kg灌胃,照射后0.5 h再次给予1.67 mg/kg尼尔雌醇灌胃。灌胃2 w后,除正常对照组外,各组均以剂量率为0.8 Gy/min的60Coγ射线全身照射一次,照射剂量为5.0 Gy。于辐照后3 d、14 d测定小鼠体重、外周血WBC,辐照后14 d测定小鼠胸腺指数,脾指数,骨髓细胞和脾细胞DNA含量,血清SOD和MDA含量。结果γ射线全身照射后第3天,方格星虫多糖低、中剂量组小鼠外周血WBC数明显升高（P〈0.05）。照后第14天,低剂量组小鼠外周血WBC数显著增高（P〈0.01）,中、高剂量组脾脏重量指数明显升高（P〈0.01和P〈0.05）,中剂量组脾脏DNA含量明显增高（P〈0.01）;低、中剂量组骨髓细胞DNA含量明显增高（P〈0.05）;低、中、高三个剂量组小鼠血清SOD活力明显增强,血清MDA含量明显下降（P〈0.05）。结论方格星虫多糖对亚致死剂量γ辐射损伤小鼠具有一定保护作用。

Protective Effect of Sipunculus Nudus Polysaccharides on 5. 0 Gy ~（60）Coγ Irradiation Mice

Objective To evaluate the protective effects of sipunculus nudus polysaccharides（ SNPS） on mice with irradiation damage induced by 5. 0 Gy60Co γray. Methods 60 mice were divided into control group,model group,nilestriol group and 100 mg / kg·d,200 mg / kg·d,and 400 mg / kg·d SNPS groups. SNPS was administrated by intragastric infusion every day,and nilestriol was administrated by intragastric infusion with 3. 3 mg / kg,1. 67 mg / kg in the day before and after iradiation,respectively. In the fifteenth day,the mice except control group were irradiated by γrays of60Co（ 0. 80 Gy / h） with the dose of 5. 0 Gy. Peripheral blood WBC,the spleen index（ SI）,thymus index（ TI）,DNA contain in spleen cells and in bone marrow cells,SOD and MDA in serum were detected. Results Peripheral blood WBC in 100mg / kg·d and 200 mg / kg·d SNPS groups were higher than that of model group at the third day after irradiation（ P 0. 05）. Peripheral blood WBC in 100mg / kg·d,SI in 200 mg / kg·d and 400 mg / kg·d SNPS groups,DNA contain in spleen cells in 200 mg / kg·d SNPS group,DNA contain in in bone marrow cells in 100 mg / kg·d and 200 mg / kg·d SNPS groups,and SOD in serum in three SNPS groups were much higher than that of model group. MDA in three SNPS groups were much lower than that of model group at the fourteenth and third day after irradiation. Conclusion SNPS has radiation protective effects on γrays irradiation mice.