建立评价当归质量的HPLC指纹图谱分析方法

 目的建立评价当归质量优劣的指纹图谱分析方法。方法采用高效液相色谱分析，Hypersil C₁₈柱(200 mm×4.6 mm，5μm)，分别以乙腈-0.01%磷酸水溶液(10∶90)、甲醇-乙腈-水(10∶30∶60)为流动相，流速为0.8 mL·min^-1，检测波长320 nm，测定了36批不同产地的当归药材的HPLC指纹图谱。结果 根据聚类分析和相似度分析处理结果，将当归药材分为两类：合格药材与不合格药材。结论 本方法简便、快捷、可靠，可用于当归的质量评价。

Establishment of HPLC-fingerprint analysis for the quality assessment of Angelica sinensis

OBJECTIVE To establish the HPLC-fingerprint analysis for the quality control of Angelica sinensis. METHODS The HPLC method was used with Hypersil C₁₈column(200 mm×4.6 mm，5μm).The mobile phases were consisted of acetonitrile 0.01% phosphoric acid (10∶90) and methanol acetonitrile water (10∶30∶60) respectively.The flow rate was 0.8 mL·min^-1.The UV detection was performed at 320 nm.36 batches of Angelica sinensis were determined. RESULTS The 36 batches of Angelica sinensis were classified to be qualified and unqualified based on the results of cluster analysis and similarity analysis. CONCLUSION The method was simple and reliable,and it was capable of effectively controlling the quality of Angelica sinensis.