荧光原位杂交（FISH）检测乳腺癌中 HER －2基因状态与 p53、Ki －67、TOPOⅡ表达的相关性

目的：研究乳腺癌中 HER －2基因状态和 p53、Ki －67、TOPOⅡ蛋白表达的相互关系，以及 HER －2基因扩增与 HER －2蛋白表达的相关性。方法：运用 FISH 和 IHC 技术分别检测172例浸润性乳腺癌中 HER－2基因状态及 HER －2、p53、Ki －67、TOPOⅡ蛋白表达情况，分析 HER －2基因状态与其相互的关系。结果：172例浸润性乳腺癌中 HER －2基因扩增与 p53、Ki －67、TOPOⅡ蛋白表达呈正相关（P ＜0．05），HER －2基因扩增阳性率为43．6％（75／172），HER －2基因扩增与 HER －2蛋白表达具有相关性（P ＜0．05）。结论：联合检测 HER －2基因状态和 p53、Ki －67、TOPOⅡ蛋白表达可为浸润性乳腺癌的预后及分子靶向治疗提供依据。

HER -2 gene status and its correlation with expression of p53,Ki -67,TOPOⅡ in breast cancer

Objective:To study HER -2 gene status and its relationship with the protien expression of p53,Ki -67,TOPOⅡ in breast cancer,and the correlation between HER -2 gene amplification and its protein expression. Methods:Detected the HER -2 gene status and the protein expression of HER -2 gene,p53,Ki -67,and TOPOⅡin 172 cases of invasive breast cancer,by FISH and IHC technique,and analyze the relationship between state of HER-2 and protein expression.Results:In 172 cases of invasive breast cancer,the HER -2 gene amplification was posi-tively correlated with p53,Ki -67,TOPOⅡ protein expression(P <0.05).The positive rate of HER -2 gene amplifi-cation was 43.6%(75 /172).And HER -2 protein expression was correlated with HER -2 gene amplification(P <0.05).Conclusion:Joint detection of HER -2 gene state and protein expression of p53,Ki -67,TOPOⅡ could pro-vide basis for prognosis and molecular targeted therapy for invasive breast cancer.