塞来昔布对人鼻咽癌 CNE -2细胞放疗的增敏作用 |
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引用本文: | 李超,成浩,肖华光,王倩.塞来昔布对人鼻咽癌 CNE -2细胞放疗的增敏作用[J].现代肿瘤医学,2016(21):3359-3363. |
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作者姓名: | 李超 成浩 肖华光 王倩 |
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作者单位: | 南华大学附属郴州市第一人民医院,湖南 郴州,423000 |
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基金项目: | 湖南省卫生厅技术项目(编号湘卫科教发(2013)5号) |
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摘 要: | 目的:探讨塞来昔布对人鼻咽癌 CNE -2细胞的放射增敏作用及其可能机制。方法:四唑盐比色法(MTT)测定塞来昔布对人鼻咽癌 CNE -2细胞株的抑制率。采用克隆形成实验检测塞来昔布处理的 CNE -2细胞经不同剂量 X 线(0、2、4、6、8和10Gy)照射后的存活分数(SF),并通过单击多靶模型拟合细胞存活曲线,计算增敏比(SER)。流式细胞仪(FCM)分析细胞周期分布及凋亡率。结果:MTT 实验显示塞来昔布在(10~80)μg/ml 范围内对 CNE -2细胞有抑制作用,抑制率与浓度、时间呈依赖关系;在2~10Gy 照射范围内,10、20、40、80μg/ml 塞来昔布处理后的 SF 均低于0μg/ml(P <0.05),且 SF 随塞来昔布浓度的增加而降低;相对于0μg/ml,10、20、40、80μg/ml 塞来昔布处理后的 SER 分别为1.06、1.79、2.29和3.34;流式细胞仪测得塞来昔布可呈浓度依赖的方式诱导 CNE -2细胞凋亡(P <0.05),S 期细胞比例降低,G2/M期细胞比例升高。结论:塞来昔布能抑制人鼻咽癌 CNE -2细胞增殖,诱导 CNE -2细胞细胞周期阻滞,促进细胞凋亡,具有放疗增敏作用。
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关 键 词: | 鼻咽癌 塞来昔布 放疗增敏 凋亡 |
Radiotherapy sensitization effect of celecoxib on nasopharyngeal carcinoma cell line CNE -2 |
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Abstract: | Objective:To investigate radiotherapy sensitization effect of celecoxib on nasopharyngeal carcinoma cell line CNE -2 and its possible mechanism.Methods:MTT was applied to detect the inhibition rate of celecoxib on nasopharyngeal carcinoma cell line CNE -2.The clone formation assay was applied to investigate the survival fraction (SF)of the CNE -2 cells treated with celecoxib after different doses of X -ray(0,2,4,6,8 and 10Gy)irradiation. The sensitization enhancement ratio(SER)was calculated by cell survival curve fitted with the target model .The cell cycle distribution and apoptosis rate were detected by flowcytometry.Results:Celecoxib inhibited the growth of CNE -2 cells and it was in a dose and time -dependent manner in range of (10 ~80)μg/ml.In 2 ~10Gy irradiation range, the SF of 10,20,40,80μg/ml celecoxib were lower than that of 0μg/ml(P <0.05),and SF declined with the in-crease of concentration of celecoxib.Compared with 0μg/ml celecoxib,SER of 10,20,40,80μg/ml celecoxib after treatment were 1.06,1.79,2.29 and 3.34,respectively.Celecoxib induced the apoptosis of CNE -2 cells in a dose -dependent manner(P <0.05).Moreover,celecoxib reduced the proportion of S phase and increased the proportion of G2 /M phase.Conclusion:Celecoxib can inhibit the proliferation of nasopharyngeal carcinoma cell line CNE -2 and has the effect of radiation sensitization through cell cycle blocking and acceleration of apoptosis. |
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Keywords: | nasopharyngeal carcinoma celecoxib radiotherapy sensitization apoptosis |
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