低温等离子体对天然胶原材料表面改性的研究

采用牛腱中提取的胶原蛋白为原料，制成薄膜试样，在Ｏ２和Ａｒ条件下，用低温等离子体处理天然胶原薄膜的表面．处理后的试样通过电子光谱（ＥＳＣＡ），红外光谱（ＩＲ），Ｘ射线衍射和接触角测量来研究其结构和性能的变化．实验表明：处理后胶原材料的表面、内部结构及化学成分都发生了明显的变化；它与水和二碘甲烷的接触角明显小于等离子处理前的可见低温等离子体方法能用于胶原材料的改性．

THE EFFECT OF LOW TEMPERATURE PLASMA ON SURFACE OF NATURAL COLLAGEN

The surface of natural collagen was modified by low temperature plasma with oxygen and argon seperately. Natural collagen was extracted and purified from bovine tendon by enzyme digestion.Crosslinked collagen membranes were then prepared from a 0.8% (w / v) solution in 0. 3% malonic acid.After treating by low temperature plasma, their surface characteristics were investigated by means of ESCA,IR, XRD and contact angle measurement. The results of ESCA and IR shows that the ratios of main functional groups upon plasma modified collagen surface such as carbonyl- (> C = O), carboxyl- (-COOH),amino- (NH2) and hydroxyl- (-OH) groups have changed significantly. The results of XRD also suggest the occurence of considerable structural changes.With water and CH2I2, contact angles decrease and surface energies increase after modification. The conclusion is that low temperature plasma treatment can substantially alter the surface properties of collagen material by ways of increasing the amount of polar functional groups exposed on the sample surface thus enhancing its hydrophilicity.Correspondent ZHANG Qiqing, Professor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences,P.O. Box 25 (204), Tianjin 300192