坚杆火绒草中总酚酸的提取及含量测定

目的:考察不同采收期坚杆火绒草不同部位中总酚酸含量的差异,验证"坚杆火绒草的采收时间为花期,使用部位为叶子和花"的合理性。方法:以绿原酸为对照品,以福林酚为显色剂,检测波长765 nm,采用紫外-可见分光光度法测定总酚酸含量。结果:绿原酸吸光度与质量浓度在0~20 mg·L-1线性良好(R2=0.999 7),平均加样回收率95.8%(RSD 1.0%)。坚杆火绒草花期的叶中总酚酸提取量高达190.606 mg·g-1;花期总酚酸含量明显高于果期,同一植株叶子和花中总酚酸含量明显高于其他部位。结论:总酚酸可作为坚杆火绒草最佳采收期及药用部位的评价指标,为该药材的质量控制提供参考。

Extraction and Determination of Total Phenolic Acids in Leontopodium franchetii at Different Harvest Time

Objective: To verify that acquisition time of Leontopodium franchetii was florescence and application parts were leaf and flower by investigating content differences of total phenolic acids in L. franchetii at different harvesting time. Method: UV-Vis spectrophotometry was adopted to determine the content of total phenolic acids with chlorogenic acid as standard,Folin-phenol as chromogenic agent and detection wavelength at 765 nm. Result: Linearity range of chlorogenic acid was 0-20 mg·L^-1(R²=0.999 7),average recovery was 95.8% with RSD of 1.0%.The content of total phenolic acids from leaves of L. franchetii was up to 190.606 mg·g^-1 in florescence.It showed that the content of total phenolic acids at florescence was significantly higher than other periods in L. franchetii,it in leaves and flowers were significantly higher than other plant parts. Conclusion: Total phenolic acids can be used as an evaluation index to confirm optimum acquisition time and effective parts of L. franchetii.It provides scientific basis for rational utilization and quality control of L. franchetii.