沙棘叶中绿原酸提取工艺研究

在单因素试验的基础上,采用正交试验的方法,对水煎煮法和酶解法提取沙棘叶中绿原酸的工艺条件进行了优化。试验结果表明:水煎煮法最佳提取工艺条件为:料液比(g∶mL)1∶35,煎煮时间1.0 h,煎煮次数3次。该条件下,沙棘叶中绿原酸提取率为2.42%。酶解法最佳提取工艺条件为:以加5%纤维素酶的pH 4.5的酸性水为提取剂,料液比(g∶mL)1∶15,温度50℃,提取时间1.0 h。该条件下,绿原酸提取率为3.49%。酶解法与水煎煮法相比,绿原酸提取率提高了44.2%。

Study on Extraction Procedure of Chlorogenic Acid from Seabuckthorn Leaves

Optimum conditions of extraction chlorogenic acid from seabuckthorn leaves with water decocting and enzymolysis method were studied by the single factor experiment and orthogonal experimental.The results showed that: Optimum extraction conditions of water decocting method were as follows: the ratio of materials to solution was 1∶35(g∶mL) and decocting time 1.0 h with 3 times.Through such technical process,the extraction rate of chlorogenic acid was 2.42%.Optimum extraction conditions of enzymolysis were as follows: the acidic solution(pH 4.5) added 5% cellulase as extractant,the ratio of materials to solution was 1∶15(g∶mL),extraction temperature was 50 ℃,and extraction time was 1.0 h.At such condition,the extraction rate of chlorogenic acid was 3.49%,which increased by 44.2% compared with decocting method.