针药结合对脑缺血大鼠下丘脑室旁核脑源性神经营养因子和血管内皮生长因子表达的影响

目的:观察电针、天麻多糖及电针结合天麻多糖对脑缺血大鼠下丘脑室旁核(PVN)脑源性神经营养因子(BDNF)和血管内皮生长因子(VEGF)表达的影响,探讨针药结合治疗脑缺血的可能机制。方法:将SD大鼠随机分为正常组、模型组、电针组、天麻多糖组和电针结合天麻多糖组,每组8只。以线栓法制备脑缺血模型。造模成功后,电针组和电针结合天麻多糖组大鼠给予"百会""足三里"穴电针刺激,30min/次,每天1次,连续14d;天麻多糖组和电针结合天麻多糖组大鼠每天给予天麻多糖100mg/kg灌胃,连续14d。采用免疫组化法观察各组大鼠PVN的BDNF和VEGF表达变化。结果:模型组缺血侧PVN的BDNF、VEGF阳性表达较正常组增加(P0.05);电针组、天麻多糖组和电针结合天麻多糖组缺血侧PVN的BDNF、VEGF阳性表达均较模型组增加(P0.05);电针结合天麻多糖组PVN的BDNF、VEGF阳性表达较电针组或天麻多糖组增加(P0.05)。结论:电针结合天麻多糖疗法对大鼠脑缺血损伤有保护作用,其机制可能与脑缺血大鼠缺血侧PVN的BDNF、VEGF表达增加有关,且效果优于单用电针或天麻多糖。

Effect of Electroacupuncture Combined with Polysaccharide of Gastrodia elata Blume on Expression of Brain Derived Neurotrophic Factor and Vascular Endothelial Growth Factor in the Paraventricular Nucleus of Hypothalamus in Cerebral Ischemia Rat

Objective To observe the effect of electroacupuncture(EA)of"Baihui"(GV 20)and"Zusanli"(ST 36)in combination with administration of polysaccharide of Gastrodia elata Blume(PGB)on the expression of brain derived neurotrophic factor(BDNF)and vascular endothelial growth factor(VEGF)in the paraventricular nucleus of hypothalamus(PVN)of cerebral ischemia(CI)rats,so as to explore its mechanism underlying improvement of CI.Methods Forty Sprague-Dawley rats were randomized into five groups:normal control,model,EA,PGB,and EA+PGB(n=8/group).The cerebral ischemia model was established by occlusion of the middle cerebral artery(MCAO).EA(2Hz,2V)was applied to"Baihui"(GV 20)and"Zusanli"(ST 36)for 30 min,once daily for 14 days.The rats of the PGB and PGB+EA groups were treated by intragastric gavage of PGB at a dose of 100mg/kg,once daily for 14 successive days.The expression of BDNF and VEGF in the PVN of hypothalamus was detected by immunohistochemistry.Results After MCAO,the BDNF and VEGF immunoreactions(IR)positive neuron numbers and expression level in the PVN of the hypothalamus were significantly increased in the model group(P<0.05)and were further up-regulated after administration of EA and PBG(P<0.05).The effects of EA+PGB were evidently superior to those of simple EA and simple PBG in up-regulating BDNF and VEGF IR-positive neuron numbers and expression levels in the PVN(P<0.05).Conclusion EA combined with PGB can up-regulate the expression of BDNF and VEGF in the PVN of hypothalamus in cerebral ischemia rats,which might contribute to its effect in improving cerebral ischemia.