Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water |
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Authors: | P Walther & A Ziegler |
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Affiliation: | Zentrale Einrichtung Elektronenmikroskopie, Universitaet Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany |
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Abstract: | Biological membranes are often poorly visible with the electron microscope after high‐pressure freezing and freeze‐substitution. The water content of the sample and of the substitution medium is one factor among others that strongly influences membrane visibility. In order to investigate this effect, high‐pressure frozen yeast cells, rat‐pancreas tissue and arthropod tissue were freeze‐substituted with and without adding water to the substitution medium. The visibility of the biological membranes was generally improved if the substitution medium contained 1–5% water. The effect was especially pronounced in yeast cells, where membrane visibility was poor after freeze‐substitution with water‐free medium but good after addition of 5% water to the substitution medium. |
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Keywords: | Cryo-preparation electron microscopy freeze substitution high pressure freezing membranes TEM yeast |
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