Recombinant Trypanosoma cruzi antigens and Chagas' disease diagnosis: analysis of a workshop |
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| Authors: | MJ Levin J Franco da Silveira ACC Frasch ME Camargo S Lafon WM Degrave R Rangel-Aldao |
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| Affiliation: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Buenos Aires, Argentina;Disciplina de Parasitología, Escola Paulista de Medicina, Sao Paulo, Brazil;Instituto de Investigaciones Bioquímicas, Fundación Campomar, Buenos Aires, Argentina;Laboratório Fleury, Sao Paolo, Brazil;Departamento de Biología Molecular Fundación Oswaldo Cruz, Rio de Janeiro, Brazil;Universidad Simón Bolivar, Caracas, Venezuela |
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| Abstract: | Abstract A workshop organized by the Ibero-American Project of Biotechnology evaluated the diagnostic potential of several cloned Trypanosoma cruzi recombinant antigens for Chagas' disease serodiagnosis. A set of recombinants, Antigen 2, Antigen 13, SAPA, H49, A13, JL5, JL7, JL8, JL9, and RA1 provided by three different South American laboratories were probed with a panel of 236 South American serum samples. Antigens JL7, H49, Antigen 2, and A13 scored as the best diagnostic recombinant reagents. The results suggested that the main advantage of using cloned peptides for chronic Chagas' disease diagnosis resided in their highly specific immunoreactive properties. |
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| Keywords: | Trypanosoma cruzi Diagnosis Chagas' disease Trypanosoma cruzi recombinant antigens Immunoassay |
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