不同数量神经干细胞移植治疗实验性脑梗死

背景:研究已经确证脑梗死后进行神经干细胞移植可以促进动物神经功能恢复。目的:观察在大鼠实验性脑梗死后移植不同数量神经干细胞的效果,以期寻找出能有效促进神经功能恢复的最小移植数量。方法:提取Wistar胚胎鼠脑组织,体外培养神经干细胞。利用线栓法制作Wistar大鼠脑梗死模型。在大鼠脑梗死第7天,取培养第12天的神经干细胞,预标记BrdU后通过立体定向、微量注射泵方法,分别移植低6×105、中8×105、高10×105数量的神经干细胞,并设立假移植组,通过抓握牵引实验、斜板实验评价移植3个月时4组动物的行为学变化,以及BrdU、神经元特异性烯醇化酶、胶质纤维酸性蛋白等免疫组织化学染色方法检测移植后神经干细胞与周围宿主整合及向神经元、神经元胶质细胞分化情况。结果与结论:神经干细胞移植后能在宿主脑内存活,并分化为神经元和胶质细胞促进功能恢复。移植神经干细胞后3个月时,各移植组与假移植组相比行为学评分均有显著性升高;中等数量移植组大鼠各项行为学评分明显优于低等数量移植组,而中、高等数量移植组两组之间差异没有显著性意义。提示脑梗死后移植不同数量神经干细胞可改善因脑梗死造成的功能障碍,8×105数量神经干细胞移植可以较少的数量发挥较好移植效果。

Different quantities of neural stem cells transplantation in treating experimental cerebral infarction

BACKGROUND: Studies have confirmed that neural stem cells (NSCs) transplantation can promote nerve functional recovery in animals with cerebral infarction. OBJECTIVE: To observe the effect of different quantities of NSCs transplantation in rats with cerebral infarction and to find the minimum quantity of transplanted cells to promote nerve functional recovery effectively. METHODS: Embryonic brain tissues of Wistar rats were extracted and NSCs were cultured in vitro. The cerebral infarction models of Wistar rats were constructed by using suture method. At 7 days after cerebral infarction, cultured NSCs were extracted at 12 days. BrdU-labeled NSCs with 6×105, 8×105 and 10×105 were transplanted using stereotactic and micro-injection pump. A sham-transplanted group was established. Behavioral changes at 3 months after transplantation were evaluated by using prehensile traction test and inclined plane test. The differentiation of transplanted NSCs integrated with the surrounding host into neurons and glial cells was detected by using immunohistochemical staining in BrdU, neuron-specific enolase, glial fibrillary acidic protein. RESULTS AND CONCLUSION: NSCs could survive in the host brain after transplantation and differentiate into neurons and glial cells to promote functional recovery. At 3 months after NSCs transplantation, compared with the sham-transplanted group, the behavioral score was significantly increased in the transplanted group. The behavioral score was improved more significantly in the moderate quantity transplanted group than in the low quantity transplanted group, while there was no significant difference between the moderate quantity transplanted group and high quantity transplanted group. It is indicated that different quantities of NSCs transplantation in treatment of cerebral infarction can promote dysfunction recovery. The quantity of NSCs transplantation 8×105 can play better effect with less quantity transplantation