| 甜瓜叶片中Rubisco的去除及双向电泳体系的优化 |
| |
| 引用本文： | 钟俐,马成梅,梁永增,李冠.甜瓜叶片中Rubisco的去除及双向电泳体系的优化[J].植物生理学通讯,2012(3):303-309. |
| |
| 作者姓名： | 钟俐 马成梅 梁永增 李冠 |
| |
| 作者单位： | 新疆大学生命科学与技术学院,乌鲁木齐830046 |
| |
| 基金项目： | 国家自然科学基金（31060148）; 新疆维吾尔自治区自然科学基金（2011211A013）; 新疆维吾尔自治区高技术研究发展项目（201111120） |
| |
| 摘 要： | 为降低Rubisco的干扰,建立适合于甜瓜叶片蛋白质的双向电泳技术,本文比较了不同全蛋白提取方法和上样量对双向电泳的影响。结果表明,Mg/NP-40/PEG3350/TCA/丙酮提取法可去除样品中绝大多数的Rubisco,使低丰度蛋白得以检测,适合后续分析。以该法提取的蛋白,采用600、800、1000和l200μg四种上样量进行双向电泳,上样量为l000μg的样品用pH3～10的IPG胶条,在2-DE胶上分辨出质量好、数量多的蛋白质点（562个）。因此,Mg/NP-40/PEG3350/TCA/丙酮法是适合甜瓜叶片蛋白质提取的方法,l000μg是最适上样量。
|
| 关 键 词： | 甜瓜 Rubisco 蛋白质提取 双向电泳 上样量 |
Depletion of Rubisco from Cucumis melon Leaf and Optimization of Two-Dimensional Electrophoresis for Proteome |
| |
| ZHONG Li,MA Cheng-Mei,LIANG Yong-Zeng,LI Guan.Depletion of Rubisco from Cucumis melon Leaf and Optimization of Two-Dimensional Electrophoresis for Proteome[J].Plant Physiology Communications,2012(3):303-309. |
| |
| Authors: | ZHONG Li MA Cheng-Mei LIANG Yong-Zeng LI Guan |
| |
| Affiliation: | ＊College of Life Science and Technology,Xinjiang University,Urumqi 830046,China |
| |
| Abstract: | This research compared the effects of different methods of protein extraction of Cucumis melon leaves and different loading amounts on 2-DE profile.The results indicated that Mg/NP-40/PEG3350/TCA/acetone precipitation was a suitable method for subsequent analysis attributed to reducing the interference of Rubisco.There were 562 proteins could be well resolved and detected in the gels when l 000 μg protein of leaves was loading with pH 3-10 IPG strips.All the results showed that Mg/NP-40/PEG3350 TCA/acetone precipitation was much more suitable for protein extraction and l 000 μg was the optimum loading amount of Cucumis melon leaves. |
| |
| Keywords: | Cucumis melo L Rubisco protein extraction two-dimensional electrophoresis（2-DE） loading quantity of sample |
| 本文献已被 维普 等数据库收录！ |
