转TPSP融合基因小麦的耐旱相关特性

在获得转TPSP基因小麦纯合株系的基础上,对3个转基因株系的耐旱相关生理特性进行了分析。脯氨酸含量测定显示,干旱胁迫过程中小麦叶片中脯氨酸含量逐渐增加,且3个转基因株系叶片中脯氨酸的积累速度和积累量均显著高于非转基因对照;叶绿素荧光参数测定显示,3个转基因株系的Fv/Fm值在胁迫过程中均略高于非转基因对照,转基因株系4-4-4的Fv/Fo值显著高于非转基因对照,表明转基因株系在水分胁迫条件下光合系统II（PSII）的光合效率有所增强;转基因小麦耐旱性鉴定显示：模拟干旱胁迫100h时对照小麦叶片几乎全部萎蔫,而3个转基因株系均表现出较强的耐旱性;复水24h后转基因株系4-9-1、4-4-4和30-1-2的叶片黄化率分别为25.2%、23.3%和27.6%,显著低于非转基因对照（48.8%）。上述研究结果表明转TPSP基因小麦具有较强的耐旱能力,为转基因材料进一步应用于小麦抗旱育种提供了依据。

The Drought Tolerance Related Characteristics of Transgenic Wheat with the TPSP Fusion Gene

Based on the transgenic homozygous lines of wheat with the TPSP fusion gene,the drought tolerance related characteristics of 3 transgenic lines were analyzed.Comparative analysis on proline content in wheat leaves showed a gradually increasing pattern during the drought stress,and the accumulating speed and volume of praline in 3 transgenic lines were significantly higher than that in no-transgenic control.The detection of chlorophyll fluorescence parameters showed that the Fv/Fm values of 3 transgenic lines were slightly higher than that of no-transgenic control under the drought stress,while the Fv/Fo values of transgenic line 4-4-4 were significantly higher than that of control,which indicated that the photosynthetic efficiency of photosystem II in transgenic lines were enhanced under drought stress.The drought tolerance assay using an artificial water-stress system indicated that almost all of no-transgenic leaves were severely withered at the time point of 100 h after stress treatment,while the transgenic lines presented stronger drought tolerance.The percentage of yellow leaves were calculated at the time point of 24 h after rewatering and the results showed that the yellow leaf ratios of transgenic line 4-9-1,4-4-4 and 30-1-2 were 25.2%,23.3% and 27.6%,which were significantly lower than the ratio（48.8%） of no-transgenic control.All these results indicated that the drought tolerance of transgenic wheat with TPSP gene was greatly enhanced and this provide an important basis for the further application of these transgenic materials in drought resistant breeding of wheat.