蛋用鹌鹑的VIPR-1基因的多态性与早期生长性状的关联分析

为分析VIPR-1基因是否能作为鹌鹑早期生长性状的相关分子标记或者候选基因,以中国黄羽鹌鹑、中国白羽鹌鹑、朝鲜鹌鹑3个蛋用鹌鹑品种作为实验对象,采用PCR-RFLP技术和测序技术检测VIPR-1基因的外显子6-7的多态性,并分析该基因多态性与蛋用鹌鹑早期生长性状的关联程度。结果表明,在3个蛋用鹌鹑品种中的VIPR-1基因外显子6-7区域共检测出15个SNP位点,分别为A94G、G97T、C201T、A204G、A233G、C260G、A299T、C354T、A355G、C378T、C407G、A425C、A543G、G585T、A586G。对HpyCH4IV位点(A355G)进行酶切分析,可以检测到3种基因型即AA(285 bp/811 bp)、AG(285 bp/811 bp/1 096 bp)和GG(1 096 bp)。中国黄羽鹌鹑和中国白羽鹌鹑的AA、AG、GG基因型频率分别为0.227、0.636、0.136和0.087、0.609、0.304。朝鲜鹌鹑AG和GG基因型频率分别为0.235和0.765。1~4周龄时,VIPR-1基因的HpyCH4IV位点与中国黄羽鹌鹑的日增重、体重、胫长、胸宽、胸深、胸骨长、胫围有显著关联性(P<0.05)。HpyCH4IV位点与中国白羽鹌鹑的胫围有显著关联性(P<0.05)。HpyCH4IV位点与朝鲜鹌鹑的体重、胸宽、胸深有显著关联性(P<0.05)。5~7周龄时,VIPR-1基因的HpyCH4IV位点与中国黄羽鹌鹑的体重、胸骨长、胫长、体长、胸宽、胸深、胫围、日增重和相对生长率均有显著的关联性(P<0.05)。HpyCH4IV位点与中国白羽鹌鹑的日增重和相对生长均有显著的关联性(P<0.05)。HpyCH4IV位点与朝鲜鹌鹑的体重有显著的关联性(P<0.05)。综上研究表明,VIPR-1基因外显子6-7的HpyCH4IV位点(A355G)对蛋用鹌鹑的早期生长性状具有一定影响。

Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail

To analyze whether VIPR-1 gene can be used as a molecular marker or candidate gene for early growth traits of quail or not, in this study, three egg quails, namely Chinese yellow quail, Chinese white quail and Korean quail, were used as experimental materials. PCR-RFLP and sequencing were used to detect the polymorphism of exon 6-7 of VIPR-1 gene, and the correlation between the polymorphism and the early growth traits of egg quail was analyzed. The results showed that 15 SNP loci were detected in exon 6-7 of VIPR-1 gene in three egg quails, which were A94G, G97T, C201T, A204G, A233G, C260G, A299T, C354T, A355G, C378T, C407G, A425C, A543G, G585T, A586G. Three genotypes, AA (285 bp/811 bp), AG(285 bp/811 bp/1 096 bp) and GG(1 096 bp), were detected by restriction enzyme analysis of HpyCH4IV (A355G). The genotypic frequencies of AA, AG and GG were 0.227, 0.636, 0.136, 0.087, 0.609, 0.304, respectively. The frequencies of AG and GG genotypes in Korean quail were 0.235 and 0.765, respectively. At the age of 1-4 weeks, the HpyCH4IV locus of VIPR-1 gene was significantly correlated with the daily gain, body weight, tibial length, chest width, chest depth, sternal length and tibial circumference of Chinese yellow quails (P<0.05). There was a significant correlation between HpyCH4IV locus and tibial circumference of Chinese white quails (P<0.05). There was a significant correlation between HpyCH4IV locus and body weight, chest width and chest depth of Korean quail (P<0.05). At the age of 5-7 weeks, the HpyCH4IV locus of VIPR-1 gene was significantly correlated with the body weight, sternal length, tibial length, body length, chest width, chest depth, tibial circumference, daily gain and relative growth rate of Chinese yellow quails (P<0.05). There was a significant correlation between HpyCH4IV locus and daily gain and relative growth of Chinese white quails (P<0.05). There was a significant correlation between HpyCH4IV locus and body weight of Korean quail (P<0.05). In conclusion, the HpyCH4IV locus (A355G) in exon 6-7 of VIPR-1 gene has some influence on the early growth traits of quail.