| 广金钱草及其煎剂HPLC指纹图谱的相关性研究 |
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| 引用本文： | 张素中,黄月纯,曾彩芳,陈慕媛,刘翠玲,冯小权.广金钱草及其煎剂HPLC指纹图谱的相关性研究[J].现代中药研究与实践,2010(5):76-78. |
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| 作者姓名： | 张素中 黄月纯 曾彩芳 陈慕媛 刘翠玲 冯小权 |
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| 作者单位： | [1]中山大学新华学院,广东广州510520 [2]广州中医药大学第一附属医院,广东广州510405 [3]广州医学院港湾医院,广东广州510700 |
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| 基金项目： | 广东省科技计划项目（2009B060700012）;广东省科技计划项目（2007B060401010）; 广东省中医药管理局课题（2009256） |
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| 摘 要： | 目的建立广金钱草HPLC指纹图谱分析方法,研究金钱草饮片及其煎剂指纹图谱的相关性。方法采用HPLC法分析。结果广金钱草及其煎剂均标示出11个共有峰,主要特征峰的提取率在53.39%~87.40%之间;饮片与煎剂之间的相似度为0.981~0.997之间。结论本法准确可靠,重复性好,为广金钱草的物质基础研究提供了一定参考。
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| 关 键 词： | 广金钱草 煎剂 指纹图谱 HPLC |
Analysis on Correlation of HPLC Fingerprint on the Decoction Pieces and Decoction of Desmodii Styracifolii Herba |
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| ZHANG Su-zhong,HUANG Yue-chun,ZENG Cai-fang,CHEN Mu-yuan,LIU Cui-ling,FENG Xiao-quan.Analysis on Correlation of HPLC Fingerprint on the Decoction Pieces and Decoction of Desmodii Styracifolii Herba[J].Research and Practice on Chinese Medicines,2010(5):76-78. |
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| Authors: | ZHANG Su-zhong HUANG Yue-chun ZENG Cai-fang CHEN Mu-yuan LIU Cui-ling FENG Xiao-quan |
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| Affiliation: | 1.Xinhua College of Zhongshan University,Guangzhou 510520,China;2.The Frist Affi liated Hospital of Guangzhou University of TCM,Guangzhou 510405,China;3.GangWang Hospital of Guangzhou Medical College,Guangzhou 510700,China) |
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| Abstract: | Objective To establish the method of fingerprint analysis on Desmodii Styracifolii Herba,and study the correlation of HPLC fingerprint on the decoction pieces and decoction.Methods HPLC with Zorbax SB-Aq was used,acetonitrile-0.05% phosphoric acid（gradient elution）as a mobile phase,detection wavelength was at 270 nm,flow rate was 1 mL·min-1,and column temperature was 40℃.Results 11 common peaks were separated in the decoction pieces and decoction of Desmodii Styracifolii Herba.The extraction percentages of the main peaks were 53.39%~87.40%.The similarity between the decoction pieces and decoction was 0.981~0.997.Conclusion The method is reliable,accurate and can be used as a quality control method for Desmodii Styracifolii Herba.The Flavonoids of Desmodii Styracifolii Herba were extracted by decocting method,which provides a reference for the material base of Desmodii Styracifolii Herba. |
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| Keywords: | Desmodii Styracifolii Herba decoction fingerprint HPLC |
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