胃癌组织中Smad2表达及p42ErK1/p44ErK2的活性水平

目的：探讨Smad2和p42^ErK1/p44^ErK2在胃癌发生、发展中的作用。:要用免疫组化S－P法检测48例胃癌组织和48例正常胃黏膜组织中Smad2表达及p42^ErK1/p44^ErK2活性水平。结果：胃癌中Smad2的阳性率68．8％（33／48）显著低于正常胃黏膜组织97．9％（47／48）（P＜0．01）。并与胃癌的病理分期和分化有关（P＜0．05）；胃癌中p42^ErK1/p44^ErK2的阳性率81．3％（39／48）显著高于正常胃黏膜组织10．4％（5／48）（P＜0．01），与胃癌的分期、分化及淋巴结转移有关（P＞0．05）。结论：Smad2低表达可能参与了胃癌的形成过程，p42^ErK1/p44^ErK2活性水平增高可能是胃癌形成过程中多因素激活的结果。

Expression and significance of Smad2 and p42ErK1/p44ErK2 gene in gastric cancer

Purpose To investigate the role of Smad 2 gene expression and p42 ErK1 /p44 ErK2 activity in human gastrocarcinogenesis. Methods Expression of Smad 2 and p42 ErK1 /p44 ErK2 were detected in 48 cases of gastric cancer (GC) and in 48 cases of corresponding normal tissues by streptavidin peroxidase conjugation method (S P). Results Positive rate of Smad 2 (68 8%) was significantly lower in GC than that in the normal tissues (97 9%, P< 0 01) and was related to the tumor stages and differentiation ( P< 0 05). Positive rate of p42 ErK1 /p44 ErK2 was significantly higher in GC (81 3%) than that in normal tissues (10 4%, P< 0 01).No correlation were found between increased activity of p42 ErK1 /p44 ErK2 and the tumor stages, differentiation and lymphnode metastasis of GC ( P> 0 05). Conclusions Low expression of Smad 2 may play an important role in gastrocarcinogenesis. Increased p42 ErK1 /p44 ErK2 activity may be a result of being activated by many molecules in the development of gastric carcinoma.