虾青素提高血管性痴呆小鼠学习记忆能力

目的 探讨腹腔给药虾青素对于血管性痴呆(VaD)小鼠学习记忆能力的影响及其作用机制.方法 采用双侧颈总动脉夹闭法建立小鼠VaD模型后随机分成假手术组、模型组、虾青素低剂量组及虾青素高剂量组,分别给予相应形式的药物治疗.Morris水迷宫观察各组小鼠的学习记忆及空间探索能力,尼氏染色、免疫组织化学染色、Western b...

Improvement of astaxanthin on learning and memory ability of vascular dementia mice

Objective To study the effect and mechanism of astaxanthin on learning and memory ability of vascular dementia(VaD)mice.Methods The mice were used to establish VaD model by occlusion of bilateral common carotid artery.The mice were randomly divided into sham group,model group,astaxanthin low-dose group and astaxanthin high-dose group and then given corresponding forms of drug treatments.Morris water maze was used to investigate the learning,memory and space exploration abilities of mice in each group.At the same time,the pathological morphology of brain neurons,the expression of amyloid beta-peptides 42(Aβ₄₂) protein in brain and the content of interleukin(IL)-4 and IL-6 were respectively examined by Nissl staining,immunohistochemical staining,Western blotting and ELISA.Results The average escape latency of mice in the model group on the 4 th and 5 th days was significantly higher than sham-operated group(P<0.05),and the number of plateau crossings and the duration of stay decreased significantly(P<0.05).At the same time,Nissl staining showed that the neurons in the hippocampus of the model group were shrinking and disordered.The number of neurons in the model group was significantly reduced compared with the sham-operated group.The average escape latency of mice treated with different doses of astaxanthin on the 4 th and 5 th day was significantly lower than the model group(P<0.05).The number and duration of platform crossing were significantly longer than model group(P<0.05).The number of neurons in the intervention group was obviously higher than model group,and the effect was dose-dependent.In addition,immunohistochemical staining,Western blotting analysis and ELISA detection showed that the levels of Aβ₄₂ protein and IL-6 in the astaxanthin intervention group decreased significantly compared with the model group(P<0.05),while the levels of IL-4 increased significantly(P<0.05).Conclusion Astaxanthin can reduce the expression of neurotoxic protein Aβ₄₂ protein and inhibit the inflammatory response in the brain,which can protect neurons and improve the learning and memory ability of mice.