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| 中国株乙型肝炎病毒全基因组克隆的制备与鉴定 | |
| 引用本文： | 余祖江,李晓菲,阚全程,杨东亮,郝连杰.中国株乙型肝炎病毒全基因组克隆的制备与鉴定[J].郑州大学学报(医学版),2003,38(1):45-49. |
| 作者姓名： | 余祖江 李晓菲 阚全程 杨东亮 郝连杰 |
| 作者单位： | 1. 郑州大学第一附属医院感染科,郑州,450052;华中科技大学同济医学院附属同济医院临床免疫室,武汉,430030 2. 郑州大学第一附属医院药剂科,郑州,450052 3. 华中科技大学同济医学院附属同济医院临床免疫室,武汉,430030 |
| 摘 要： | 目的：扩增全长HBV DNA基因组，建立中国株HBV感染性克隆，阐述HBV基因组变异对其不同基因生物学特性影响。方法：TD－PCR和XL－PCR技术一次扩增HBV全长基因组，克隆，PCR、酶切和测序鉴定，结果：通过PCR、酶切，测序鉴定和真核细胞转染分析，获得全长HBV基因组克隆。结论：获得了HBV全基因组克隆，在真核细胞中可以表达HBsAg，为建立HBV感染性克隆奠定物质基础。 |
| 关 键 词： | 全基因组 乙型肝炎病毒 基因克隆 中国株 |
| 修稿时间： | 2002年2月21日 |
Construction and identification of HBV full-length genome clone of Chinese strain |
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| YU Zujiang,LI Xiaofei,KAN Quancheng,YANG Dongliang,HAO Lianjie.Construction and identification of HBV full-length genome clone of Chinese strain[J].Journal of Zhengzhou University: Med Sci,2003,38(1):45-49. | |
| Authors: | YU Zujiang LI Xiaofei KAN Quancheng YANG Dongliang HAO Lianjie |
| Affiliation: | YU Zujiang,LI Xiaofei,KAN Quancheng,YANG Dongliang,HAO Lianjie1) Department of Infectious Diseases,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 4500522) Laboratory of Clinical Immunity,the Affiliated Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 3 ) Department of Pharmacy,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim: To construct hepatitis B virus ( HBV) clone of Chinese strain and expatiate on how the variant of HBV genome influences biological characteristic of gene in HBV. Methods: The full-length HBV DNA was amplified with TD-PCR and XL-PCR, and then cloned them into Puc18 vector. Results: The full-length HBV clone after determination by enzyme digestion and sequence analysis was acguired. Conclusions:It showed that the expressed antigen has good antige-nisity and can give the basement for constructing the head to tail infectious HBV clone of Chinese strain. |
| Keywords: | full-length genome hepatitis B virus clone Chinese strain |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
