蒺藜总皂苷对缺氧复氧心肌细胞存活及损伤形态的影响

目的观察蒺藜总皂苷对模拟缺血再灌注心肌细胞损伤的影响。方法利用原代培养的SD乳鼠心肌细胞建立模拟心肌缺血再灌注模型，分为正常组、模型组和蒺藜总皂苷大、小剂量组，观察损伤后心肌细胞搏动频率、存活率及心肌酶的释放，并以光镜、电镜观察形态学变化。结果蒺藜总皂苷大、小剂量组模拟缺血再灌注后心肌细胞存活率均提高（P〈0．01或0．05），心肌酶释放受抑（P〈0．01或0．05），且大剂量组比小剂量组更为明显（P〈0．05）。应用蒺藜总皂苷还可改善损伤后的搏动功能（P〈0．01），减轻细胞超微结构的损害。结论蒺藜总皂苷对模拟缺血再灌注损伤心肌细胞有保护作用。

Morphological study of preventive effect of total saponin from Tribulus terrestris on injury to cardiomyocyte after hypoxia-reoxygenation

Objective To investigate the preventive effects of total saponin from Tribulus terrestris (GSTT) on cardiomyocytes subjected to low oxygen tension and re-oxygenation injury in vitro. Methods First passage of cultured cardiomyocytes was subjected to hypoxia followed by oxygenatim. The cells were divided into 4 groups, namely normal group, injury group, and large and low dose of GSTT groups. The frequency of contraction, survival rate and release of myocardial enzymes were determined. The morphologic changes were observed with light and electron microscopy. Results Both large and low dose of GSTT significantly improved the survival rate of cardiomyocytes after hypoxia and re-oxygenation injury (P<0.01, P<0.05), and the drug inhibited the release of myocardial enzymes (P<0.01, P<0.05 ). The effect of large dose was superior to that of low dose. In addition, GSTT could improve the contractile behavior of cardiomyocytes after injury (P<0.01), and it could also ameliorate the ultrastructural injuries. Conclusion GSTT could protect cultured neonatal rat cardiomyocytes from hypoxia/ reoxygenation injury.