Ferritin protein cage nanoparticles as versatile antigen delivery nanoplatforms for dendritic cell (DC)-based vaccine development |
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| Affiliation: | 1. Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy;2. Dipartimento di Scienze Biomediche e Cliniche “Luigi Sacco”, Università di Milano, Ospedale L. Sacco, Via G.B. Grassi 74, 20157 Milano, Italy;1. School of Chemical & Biomedical Engineering, Division of Bioengineering, Nanyang Technological University, 637457, Singapore;2. Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA;1. Department of Chemical Sciences, University of Naples Federico II, Napoli, Italy;2. Department of Chemistry and Pharmacy, University of Sassari, Italy;1. Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea;2. Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, Republic of Korea;3. Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea;4. Institute of Biotechnology, Chungnam National University, Daejeon, Republic of Korea |
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| Abstract: | We utilized ferritin protein cage nanoparticles (FPCN) as antigen delivery nanoplatforms for DC-based vaccine development and investigated DC-mediated antigen-specific immune responses. Antigenic peptides, OT-1 (SIINFEKL) or OT-2 (ISQAVHAAHAEINEAGR) which are derived from ovalbumin, were genetically introduced either onto the exterior surface or into the interior cavity of FPCN. FPCN carrying antigenic peptides (OT-1-FPCN and OT-2-FPCN) were effectively delivered to DCs and processed within endosomes. Delivered antigenic peptides, OT-1 or OT-2, to DCs successfully induced antigen-specific CD8+ or CD4+ T cell proliferations both in vitro and in vivo. Naïve mice immunized with OT-1-FPCN efficiently differentiated OT-1 specific CD8+ T cells into functional effector cytotoxic T cells resulting in selective killing of antigen-specific target cells. Effective differentiation of proliferated OT-2 specific CD4+ T cells into functional CD4+ Th1 and Th2 cells was confirmed with the productions of IFN-γ/IL-2 and IL-10/IL-13 cytokines, respectively.From the Clinical EditorIn this study, the authors utilized ferritin protein cage nanoparticles as antigen delivery nanoplatforms for dendritic cell-based vaccine development and investigated DC-mediated antigen-specific immune responses using strong model antigens derived from ovalbumin, suggesting potential future clinical applicability of this or similar techniques. |
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