新疆不同民族骨肉瘤患者病变组织中p53及bcl-2和增殖细胞核抗原表达的差异

背景骨肉瘤在好发于轻少年恶性骨与软组织的肿瘤中居第一位,在临床中,发现新疆少数民族中的发病率较为多见,其发病和预后在基因水平是否存在种族差异.目的观察新疆不同民族骨肉瘤发生发展中的p53,bcl-2,增殖细胞核抗原基因表达差异情况.设计非随机临床病理标本对照的实验.单位新疆医科大学病理中心免疫组织化学实验室.对象取自新疆医科大学第一附属医院及石河子大学第一附属医院病理科1984-01-01/2001-12-31手术切除的52例骨肉瘤患者组织标本(52块),男29例,女23例;其中哈族12例,维族17例,汉族23例);另取32例瘤样病变患者组织(骨纤维结构不良,纤维异常增殖症)标本(32块),其中哈族7例,维族11例,汉族14例.所取标本患者均获完全知情同意将自己的标本用于此项研究.方法实验在新疆医科大学病理中心免疫组织化学实验室进行.两种标本中p53,bcl-2和增殖细胞核抗原表达监测应用免疫组织化学ISAB法.以磷酸盐缓冲液代替一抗作空白对照,以已知阳性的肿瘤标本作阳性对照.P53蛋白和增殖细胞核抗原阳性表达在细胞核内,有明显的红棕色颗粒状染色为阳性,而Bcl-2蛋白阳性则定位于胞浆上.主要观察指标①不同民族骨肉瘤中p53,bcl-2和增殖细胞核抗原的表达结果.②p53,bcl-2,增殖细胞核抗原在骨肉瘤和骨的瘤样病变组织的表达情况.③骨肉瘤中bcl-2与p53和增殖细胞核抗原间的相关性.结果①不同民族骨肉瘤中p53,bcI-2和增殖细胞核抗原的表达结果哈族、维族及汉各之间p53,bcl-2,增殖细胞核抗原阳性率无显著差别(P＞0.05).②p53,bcl-2,增殖细胞核抗原在骨肉瘤和骨的瘤样病变组织的表达情况骨肉瘤中P53,Bcl-2蛋白、增殖细胞核抗原表达阳性率均显著高于瘤样病变组织(42.31%比3.13%,59.62%比0.75%比31.25%,P＜0.01).③骨肉瘤中bcl-2与p53和增殖细胞核抗原间的相关性骨肉瘤中bcl-2与p53之间、bcl-2与增殖细胞核抗原之间有相关关系(X2=5.8182,4.9000,P＜0.05).结论结果表明骨肉瘤组织内p53,bcl-2和增殖细胞核抗原表达与肿瘤分化程度,民族分布未见统计学差别,说明这些基因在骨肉肿瘤发生过程中具有共同调控环节,与不同民族的遗传背景关系不大.

Different expressions of p53, bcl-2 and proliferative cell nucleus antigen in osteosarcoma tissues in patients of different nationalities in Xinjiang autonomous region

BACKGROUND: Osteosarcoma is the most common type of bone tumor and generally occurs between the age of 10 to 25; moreover, in clinical practice osteosarcoma is found to occur more often in minority nationalities in Xinjiang Autonomous Region. Do its incidence and prognosis vary between nationalities at gene levelOBJECTIVE: To observe the different expressions of p53, bcl-2 and proliferative cell nucleus antigen (PCNA) gene during the development of osteosarcoma between various Xinjiang nationalities.DESIGN: Non-randomized comparative experiment taking clinical pathological specimens as subjects.SETTING: Immunohistochemical Laboratory, the Pathological Center of Xinjiang Medical University.PARTICIPANTS: Totally 52 pieces of specimen were obtained from excised osteosarcoma tissues in the Pathological Department, the First Affiliated Hospital of Xinjiang Medical University, and the Pathological Department, the First Affiliated Hospital of Shihezi University, between January 1,1984 and December 31, 2001. The 52 cases of osteosarcoma included 29cases derived from male patients and 23 from female patients; among them there were 12 cases of Kazak minority, 17 cases of Uygur minority and 23cases of Han nationality. Other 32 specimens were obtained from 32 patients with tumor-like lesions (such as osteofibrous dysplasia or fibrous dysplasia), including 7 cases of Kazak minority, 11 cases of Uygur minority,and 14 cases of Han nationality. The informed consent was obtained from the patients.METHODS: This experiment was carried out at the Immunohistochemical Laboratory, the Pathological Center of Xinjiang Medical University. LSAB method was used to detect p53, bcl-2 and PCNA expression in the two groups. The first antibody was re placed by PBS as blank control, and the available positive expression was taken as positive control. P53 protein and PCNA were observed to express in cell nucleus, appearing obvious redbrown granules with positive expression, whereas Bcl-2 protein was expressed in cytoplasm.lationship between the expression of Bcl-2, p53 and PCNA in osteosarcoma.of different nationalities: The expression of p53, bcl-2 and PCNA in Kazak minority, Uygur minority and Han nationality was not remarkable p53, bcl-2 and PCNA in osteosarcoma and bone tumor-like lesions: The expression of P53, Bcl-2 protein and PCNA in osteosarcoma was remarkably higher than that in tumor-like lesions (42.31% vs. 3.13 %, 59.62%sion of bcl-2, p53 and PCNA in osteosarcoma: There was a close correlation between bcl-2 and p53, as well as between bcl-2 and PCNA in osteosarcoma tissues (X2 =5.818 2, 4.900 0, P＜0.05).CONCLUSION: The results indicate that there was no statistical difference between various nationalities in the expression of p53, bcl-2 and PCNA, as well as osteosarcoma differentiation. Suggesting that these genes may share the common regulation during the development of osteosarcoma,which is less associated with their nationality-related hereditary background.