| 13C-PLFA分析方法及其在土壤微生物研究中的应用 |
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| 引用本文： | 陈琢玉,涂成龙,何令令.13C-PLFA分析方法及其在土壤微生物研究中的应用[J].地球与环境,2019,47(4):537-545. |
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| 作者姓名： | 陈琢玉 涂成龙 何令令 |
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| 作者单位： | 中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550081;中国科学院大学,北京100049;中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550081;贵州医科大学环境污染与疾病监测教育部重点实验室,贵阳550025;中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550081;贵州大学,贵阳550025 |
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| 基金项目： | 国家自然科学基金项目资助（41573012、41571130041、41563013）；水利部公益性项目（201401050）。 |
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| 摘 要： | 磷脂脂肪酸（PLFA）是重要的生物标志物之一，能够提供有关微生物群落结构的信息。将PLFA分析与稳定性同位素13C示踪技术结合（13C-PLFA），可以指示微生物群落对于环境变化的响应及微生物群落间的相互作用。该技术在土壤生态系统的研究中已得到广泛应用。本文主要基于13C-PLFA技术的研究现状，介绍了该技术的原理和分析方法，举例阐述了13C-PLFA技术在土壤微生物生态学中的应用，包括植物-土壤系统中碳的微生物利用、外源碳的微生物利用、污染物的微生物降解以及在甲烷氧化菌等特定微生物群体的应用。同时分析了13C-PLFA技术在应用中的优缺点，并展望了其未来应用。
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| 关 键 词： | 土壤微生物 磷脂脂肪酸 同位素标记 甲烷氧化菌 |
| 收稿时间： | 2018/10/25 0:00:00 |
| 修稿时间： | 2018/12/24 0:00:00 |
A Review on the 13C-PLFA Analysis Method and Its Application in Soil Microbial Researches |
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| CHEN Zhuoyu,TU Chenglong,HE Lingling.A Review on the 13C-PLFA Analysis Method and Its Application in Soil Microbial Researches[J].Earth and Environment,2019,47(4):537-545. |
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| Authors: | CHEN Zhuoyu TU Chenglong HE Lingling |
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| Affiliation: | 1. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China;4. Guizhou University, Guiyang 550025, China |
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| Abstract: | Phospholipid fatty acids (PLFA), which can provide information about the microbial community structure, are a group of commonly used biomarkers. The method of coupling 13C with PLFA analysis can reveal the response of microbial communities to environmental change as well as interactions between microbial communities, and has been widely used in the soil ecosystem research. This paper reviewed the research status of 13C-PLFA, introduced the principle of this technology and illustrated applications of the 13C-PLFA technique in soil microbial ecology, including microbial utilization of carbon in the plant-soil system, microbial utilization of exogenous carbon, microbial degradation of pollutants, as well as applications in the specific microbial, such as methanotrophs. In addition, this paper summarized the advantages and disadvantages of 13C-PLFA technology and looked forward to its future application. |
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| Keywords: | soil microorganism PLFA isotope labelling methanotrophs |
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