| 莫邪菊的离体培养研究初报 |
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| 引用本文： | 彭晓英,;彭尽晖,;张良波,;黄圣卓,;周朴华.莫邪菊的离体培养研究初报[J].国外医药(植物药分册),2009(4):240-243. |
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| 作者姓名： | 彭晓英 ;彭尽晖 ;张良波 ;黄圣卓 ;周朴华 |
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| 作者单位： | [1]湖南农业大学生物科学技术学院,湖南长沙410128; [2]湖南省林业科学院,湖南长沙410004; [3]湖南农业大学园艺园林学院,湖南长沙410128 |
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| 摘 要： | 目的为建立莫邪菊的离体培养体系。方法以成熟果实中的种子为外植体，探讨不同生长调节剂对离体培养中愈伤组织诱导、不定芽的分化和试管苗生根的影响。结果培养基配方为MS＋2，4-D2．0mg／L＋6-BA0．2mg／L时，种子破壳萌发率为70％，胚轴处愈伤组织诱导率为100％；MS＋6-BA2．0mg／L为合适的丛生芽诱导和增殖培养基配方；培养基为MS0时生根率为95％，生根效果好且配方简单，移栽的试管苗成活率可达95％。绪论该试验建立的离体培养体系可用于莫邪菊的离体培养。
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| 关 键 词： | 莫邪菊 离体培养 愈伤组织 不定芽 种子 |
Primary study on in vitro culture of Carpobrotus edulis |
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| Affiliation: | PENG Xiao-ying, PENG Jin-hui,ZHANG Liang-bo, HUANG Sheng-zhuo, ZHOU Pu-hua(1. Bioscienee and Biotechnology College, Hunan Agricultural University, Changsha 410128, China; 2. Hunan Academy of Forest, Changsha 410004, China; 3. College of Horticulture and Gardening, Hunan Agricultural University,Changsha 410128, China) |
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| Abstract: | Objective To establish an in vitro culture system for Carpoorotus eaulis L.Methods, Using C. edulis L. seeds in ripe fruits as explants, explore the effects of different plant growth regulators on callus induction, adventitious buds differentiation, and rooting in vitro. Results When the media was MS＋2,4-D 2.0 mg/L＋6-BA 0.2 mg/L, the germination rate was 70%, and callus induction rate was 100%. The optimum media for caespitose buds induction and proliferation was MS＋6-BA 2.0 mg/L. Both piantlets rooting rate and survival rate reached 95% when cultured on simple medium MS0. Conclusion The established in vitro culture system can be used for C. edulis L. |
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| Keywords: | Carpobrotus edulis L in vitro culture callus adventitious buds seeds |
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