不同免疫表型弥漫大B细胞淋巴瘤的抑癌基因TP53缺失分析

目的探讨不同免疫表型的弥漫大B细胞淋巴瘤(diffuse large B-cell 1ymphoma,DLBCL)抑癌基因TP53缺失情况的差异。方法根据淋巴瘤细胞免疫标志物CD10、Bcl-6及MUM-1的表达情况,将57例DLBCL石蜡包埋组织标本分为GCB(生发中心样B细胞)与non-GCB(非生发中心样B细胞)淋巴瘤两类,用荧光原位杂交(FISH)技术检测GCB与non-GCB类中TP53缺失情况。结果 24例GCB类中有4例(16.7%)TP53缺失,33例non-GCB类中有14例(42.4%)TP53缺失。二者TP53缺失率差异有统计学意义(P<0.05)。结论 non-GCB类患者TP53缺失发生率较高,TP53缺失是non-GCB类预后差的可能原因之一。FISH可以快速、准确、灵敏地检测出TP53的缺失。

Deletion of tumor suppressor P53 gene in diffuse large B-cell lymphoma with heterogeneous immunophenotype

Objective To investigate the difference of tumor suppressor P53 gene(TP53) deletion diffuse large B-cell lymphoma(DLBCL) with heterogeneous immunophenotype.Methods According to the expression levels of lymphoma cell immune markers CD10,Bcl-6 and MUM-1,57 cases of DLBCL were classified as GCB(germinal center B cell-like) group and non-GCB group(non-germinal center B cell-like).The TP53 deletion was analyzed by fluorescence in situ hybridization(FISH) in the both groups.Results The frequency of TP53 deletion in the 33 cases of non-GCB(42.4%) was significantly higher than that in the 24 GCB cases(16.7%)(P<0.05).Conclusion The non-GCB patients showed high TP53 deletion rate,and TP53 deletion may be a predictors of poor prognosis for non-GCB patients.FISH is a rapid,accurate and sensitive technique for detection of TP53 deletion.