奇正消痛贴膏对大鼠骨缺损愈合的影响

目的:观察奇正消痛贴膏对大鼠骨缺损愈合的影响.方法:造成大鼠骨缺损模型,奇正消痛贴膏2,1,0.5 g·kg-1经皮给药4周,观察给药1～4周X射线检查、血清钙(Ca)、磷(P)、碱性磷酸酶(ALP)水平测定、生物力学测定以及病理组织学检查.结果:奇正消痛贴膏2,1,0.5 g·kg-1剂量组大鼠骨缺损部位7 ～28 d的放射线检查结果显示,与模型组比较,骨密度不同程度增高,灰度增加,在药后14 d有显著性差异(P＜0.01)；血清中ALP,Ca,P水平未有显著变化,病理组织学检查显示,奇正消痛贴膏2,1,0.5 g·kg-1组7～14 d时骨小梁形成及骨板生成速度快于模型组.结论:奇正消痛贴膏对大鼠骨缺损的愈合具有促进作用.

Recovering Effect of Qizheng Plaster on the Bone Defect in Rat Model

Objective: To investigate the recovering effect of Qizheng plaster on the bone defect in rat model. Method: The rat model of bone defect was established. Qizheng plaster was percutaneously given with doses of 2, 1, 0.5 g·kg^-1 for 4 weeks. During the period, the effect was investigated by X-ray examination, serum calcium(Ca), phosphorus (P) and alkaline phosphate (ALP) were measured, and biomechanical parameters were determined, and histopathologic examination was carried out. Result: In 2,1,0.5 g·kg^-1 groups, the X-ray examination showed that of bone mineral density (BMD) in the bone defect parts was increased within 7-28 days. The gray value in the treated groups were higher than that of the model group, and had obvious difference on the 14th day (P<0.01). There were no obvious changes in the serum ALP, Ca and P. Histopathologic examination showed that the generation rate of bony trabeculae and bony spicules in the 2,1,0.5 g·kg^-1 groups were faster than that in the model group within 7-14 days. Conclusion: Qizheng plaster has recovering effect for the bone defect in rat model.