T-DNA插入麝香百合的研究

目的]获得麝香百合转基因植株。方法]采用2步外植体法和农杆菌介导的T-DNA插入技术转化麝香百合。结果]菌液浓度OD600值为0.6~0.8时浸染效果好,同时附加250 mg/L的AS可以提高转化效率,50 mg/L G418为抗性筛选最适浓度。对经过抗性筛选的百合转化植株进行PCR分子检测,部分转基因植株呈阳性,初步证明T-DNA已插入到百合基因组中。结论]该研究为利用T-DNA插入技术筛选优良的百合新品种奠定了基础。

Study on the Integration of T-DNA into Lilium longiflorum

Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb.Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method.Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6-0.8,the addition of 250 mg/L AS could increase the transformation efficiency.The optimal concentration of G418 for screening is 50 mg/L.Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR,preliminarily proving that T-DNA gene had integrated into the genome of lily.Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique.