纤维粘连蛋白对培养的自发性高血压大鼠心肌成纤维细胞增殖及胶原合成的影响

目的:观察纤维粘连蛋白(FN)对培养的SHR和WKY大鼠心肌成纤维细胞(CFb, CFb_SHR, CFb_WKY)增殖及胶原合成的影响。方法:CFb取自12周的SHR和WKY大鼠, 采用组织块贴壁法培养, 以直接细胞计数法和 ³H]-TdR掺入率反映细胞增殖, 以³H]-脯氨酸(³H]-proline)掺入率反映胶原合成。使用FN(5 μg/cm²)预先处理24孔培养板。 结果: 与0.4% FCS对照组相比, 经72 h孵育FN明显促进CFb_SHR和CFb_WKY细胞数增多, 分别为对照组的163.75%(CFb_SHR)和170.42%(CFb_WKY)。FN促进CFb_SHR和CFb_WKY ³H]-TdR掺入增加。FN促进CFb_SHR和CFb_WKY³H]-proline掺入增加。 结论:FN促进SHR和WKY大鼠的CFb增殖及胶原合成。

Effect of fibronectin on proliferation and collagen synthesis in cardiac fibroblasts from spontaneously hypertensive rats

AIM:To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFb_SHR) and WKY (CFb_WKY). METHODS:CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and³H]-TdR incorporation using 24-well plates pre-coated with 5 μg/cm² of FN. Collagen synthesis was determined by ³H]-proline incorporation. RESULTS:As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1×10⁴ cells/mL. DNA synthesis of CFb was markedly promoted by FN. FN induced an increased in ³H]-proline incorporation in both CFb_SHR and CFb_WKY. CONCLUSION:FN is able to promote cell proliferation and collagen synthesis of CFb derived both from SHR and WKY.