Transfer of Hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations |
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| Authors: | B Friebe J H Hatchett B S Gill Y Mukai E E Sebesta |
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| Affiliation: | (1) Department of Plant Pathology, Kansas State University, 66506 Manhattan, KS, USA;(2) USDA-ARS, Department of Entomology, Kansas State University, 66506 Manhattan, KS, USA;(3) USDA-ARS, Department of Agronomy, Oklahoma State University, Stillwater, OK, USA;(4) Present address: Plant Breeding Institute, Technical University of Munich, D-8050 Freising-Weihenstephan, Germany;(5) Present address: Department of Biology, Osaka Kyoiku University, Ikeda, Osaka, Japan |
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| Abstract: | Summary A new Hessian fly (Mayetiola destructor) resistance gene derived from  Balbo rye and its transfer to hexaploid wheat via radiation-induced terminal and intercalary chromosomal translocations are described. Crosses between resistant Balbo rye and susceptible Suwon 92 wheat and between the F1 amphidiploids and susceptible TAM 106 and Amigo wheats produced resistant BC2F3 lines that were identified by C-banding analysis as being 6RL telocentric addition lines. Comparative chromosomal analyses and resistance tests revealed that the resistance gene is located on the 6RL telocentric chromosome. X-irradiated pollen of 6RL addition plants was used to fertilize plants of susceptible wheats TAM 106, TAM 101, and Vona. After several generations of selection for resistance, new sublines were obtained that were homogeneous for resistance. Thirteen of these lines were analyzed by C-banding, and three different wheat-6RL chromosomal translocations (T) were identified. Wheat chromosomes involved in the translocations were 6B, 4B, and 4A. Almost the complete 6RL arm is present in T6BS · 6BL-6RL. Only the distal half of 6RL is present in T4BS · 4BL-6RL, which locates the resistance gene in the distal half of 6RL. Only a very small segment (ca 1.0  m) of the distal region of 6RL is present in an intercalary translocation (Ti) Ti4AS · 4AL-6RL-4AL. The 6RL segment is inserted in the intercalary region between the centromere of chromosome 4A and the large proximal C-band of 4AL. The break-points of the translocations are outside the region of the centromere, indicating that they were induced by the X-ray treatment. All three translocations are cytologically stable and can be used directly in wheat breeding programs.Cooperative investigations of the Kansas Agricultural Experiment Station, Departments of Entomology and Plant Pathology, the Wheat Genetics Resource Center, Kansas State University, and the US Department of Agriculture, Agricultural Research Service. Contribution No. 91-117-JDeceased |
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| Keywords: | Hessian fly resistance Insect antibiosis Wheat-rye hybrids C banding |
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