胶体金免疫层析法检测血清癌胚抗原

建立一种快速、简易的检测血清中癌胚抗原(CEA)的胶体金免疫层析法(GICA)。采用柠檬酸三钠还原法制备胶体金颗粒，标记抗CEA单克隆抗体4A3。将单克隆抗体3B5划线包被于硝酸纤维素膜上，制成免疫层析检测试纸条。血清中CEA与试纸条上胶体金标记物结合后沿着硝酸纤维素膜移动，与膜上的包被抗体结合形成肉眼可见的红色线条。用GICA与ELISA试剂盒对比检测了637份血清标本，其灵敏度为96．69％，特异性为99．38％，两法符合率为98．74％。GICA检测血清中的CEA特异性强、灵敏度高、简便快速，无需特殊仪器设备，有广泛应用价值。

Colloidal Gold Immunochromatographic Assay for Carcinoembryonic Antigen in Serum

The objective was to develop a rapid and simple colloidal gold immunochromatographic assay(GICA) for the detection of CEA in serum. Collodial gold was coupled with monoclonal anti-CEA to prepare a GICA apparatus. The gold-anti-CEA conjugate reacted with CEA in serum and migrates along the nitrocellose sirip on which anti-CEA was immobilized, thus producing the detection reaction. This method was compared with routine ELISA by testing 637 serum samples.The sensitivity was 96.69%, the specificity was 99.38% and the corresponding rate was 98.74%.The method is sensitive, specific, simple, rapid and useful for the detection of CEA in serum.