急性淋巴细胞白血病的免疫分型及CD4+CD25+T调节细胞检测

背景：在急性淋巴细胞白血病发病过程中，CD4⁺CD25⁺T调节细胞对机体免疫反应可能起着一定的调节作用。 目的：观察急性淋巴细胞白血病患者的免疫分型及外周血CD4+CD25+T调节细胞的变化情况。 方法：采用流式细胞仪对35例急性淋巴细胞白血病患者进行免疫分型，并检测外周血CD4⁺CD25⁺T调节细胞的数目，与18名健康对照作比较。 结果与结论：急性B细胞淋巴细胞白血病22例，急性T细胞淋巴细胞白血病13例；22例急性B细胞淋巴细胞白血病中CD19的阳性表达率最高(100%)，而13例急性T细胞淋巴细胞白血病中CD7阳性表达率最高(100%)。急性B细胞淋巴细胞白血病患者外周血CD4⁺CD25⁺T调节细胞和急性T细胞淋巴细胞白血病患者差异无显著性意义(P > 0.05)，但均高于健康对照(P < 0.05)。表明急性B细胞淋巴细胞白血病中CD19阳性表达率最高，急性T细胞淋巴细胞白血病中CD7阳性表达率最高，同时急性淋巴细胞白血病患者外周血CD4⁺CD25⁺T调节细胞水平显著增高。

Immunophenotype of acute lymphoblastic leukemia and detection of CD4+CD25+ regulatory T cells

BACKGROUND: CD4⁺CD25⁺ regulatory T cells (Treg) may have a certain regulatory effect on immune response in the pathogenic process of acute lymphoblastic leukemia (ALL). OBJECTIVE:To explore the immunophenotype and the changes of Treg in the peripheral blood of patients with ALL. METHODS:The immunophenotype and levels of CD4⁺CD25⁺Treg in the peripheral blood were detected using flow cytometry in 35 ALL patients in comparison with the health control people. RESULTS AND CONCLUSION:There were 22 B-ALL cases and 13 T-ALL cases among 35 ALL patients. CD19 expression of the cases of B-ALL had the highest positive rate (100%); meanwhile, CD7 expression of the cases of T-ALL had the highest positive rate (100%). There were no significant difference between the number of CD4⁺CD25⁺ Treg cells in B-ALL and T-ALL patients (P > 0.05), but there were significant difference between the number of Treg cells in health control people and ALL patients (P < 0.05). The results show that CD19 expression of the cases of B-ALL and CD7 expression of the cases of T-ALL had the highest positive rate; the levels of CD4⁺CD25⁺ Treg cells significantly increased in the peripheral blood of ALL patients.