植物乳杆菌发酵培养基的优化及其高密度培养技术

应用响应面法优化植物乳杆菌培养基配方以及利用中和法与指数流加法优化植物乳杆菌高密度培养的发酵条件。在单因素试验基础上，进一步采用SAS软件进行中心组合设计和响应面法优化发酵培养基。优化后的培养基配方为：葡萄糖质量分数5.43%、蛋白胨质量分数0.98%、K2HPO4质量分数0.59%。利用15L全自动发酵罐，在接种量3%、pH6.5、培养温度35℃的最佳条件下，采用氨水中和发酵培养基和指数流加碳、氮源，最终发酵液中植物乳杆菌菌体浓度达到9.3×109CFU/mL。

Lactobacillus plantarum: Optimization of Fermentation Medium and Investigation of High-density Culture Methods

In this study, response surface methodology was applied to optimize medium composition for Lactobacillus plantarum, and high-density cultivation of L. plantarum was optimized by neutralization and exponential feeding method. On the basis of single-factor experiments, the optimal fermentation medium formula was determined by central composite design combined with response surface methodology to be made up of 5.43% glucose, 0.98% peptone and 0.59% K2HPO4. Ammonia neutralization and exponential feeding carbon and nitrogen sources together operated in a 15 L fermenter under the optimal conditions (inoculation amount of 3%, pH 6.5 and cultivation temperature of 35 ℃) resulted in a final cell concentration of up to 9.3×109 CFU/mL.