河南省骨髓库汉族捐献者HLA-A、B、DR高分辨基因多态性调查分析

背景：2009年开始，中华骨髓库加大HLA高分检测的力度，可缩短配型检索周期，提高配型成功率。 目的：统计分析河南省骨髓捐献者HLA-A、B、DRB1高分辨基因多态性。 方法：河南省汉族造血干细胞志愿捐献者血样3 874人份，志愿捐献静脉血，EDTA抗凝。采用SSO HD 荧光微珠流式高分辨HLA-A、B、DRB1基因分型检测方法，对3 874份骨髓捐献志愿者血样进行高分辨分型检测，用PCR-SSP高分辨检测、SBT检测解决存在的模棱两可问题。等位基因计数法计算等位基因的基因频率。 结果与结论：共检出A 34个，B 84个，DRB1 50个，A位点基因频率处于前5位的有A*0201(0.160 9)、 A*1101(0.162 7)、 A*2402(0.160 2)、A*3001(0.080 8)、 A*3303(0.078 9)；B位点处于前5位的是B*1302(0.077 7)、B*4001(0.072 2)、B*5101(0.062 8)、B*4601(0.061 2)和B*0702(0.050 7)；DRB1位点处于前5位的是DRB1*1501(0.146 9)、DRB1*0901(0.131 8)、DRB1*0701(0.121 1)、DRB1*1202(0.061 2)、DRB1*0405(0.058 2)。

HLA-A,B and DR high-resolution gene polymorphism of Henan Stem Cell Registry

BACKGROUND:Since 2009, Chinese Marrow Donor Program takes more efforts in high resolution HLA typing, shortens typing retrieval time and increases the success rate of HLA typing. OBJECTIVE:To statistically analyze HLA-A, B, DRB1 high-resolution gene polymorphism in bone marrow donors from Henan Province, China. METHODS:A total of 3 874 venous blood samples were collected from the volunteer donors and subjected to ethylenediamine tetraacetic acid anticoagulation. The blood samples were detected using PCR-SSO HD high-resolution HLA typing method (LABType ® HD, Onelambda USA). The SSP and SBT detection tests were performed to solve ambiguity. Allele frequency was calculated with the direct count method. RESULTS AND CONCLUSION:A total of 168 alleles were detected, 34 of which were for HLA-A, 84 for B, and 50 for DRB1. The top five loci for each was A*0201(0.160 9), A*1101 (0.162 7), A*2402(0.160 2), A*3001(0.080 8), A*3303 (0.078 9); B*1302(0.077 7), B*4001(0.072 2), B*5101(0.062 8), B*4601(0.061 2), B*0702(0.050 7); DRB1*1501(0.146 9), DRB1*0901(0.131 8 ), DRB1*0701(0.121 1), DRB1*1202(0.061 2), DRB1*0405(0.058 2).