工业化UASB厌氧颗粒污泥产氢产乙酸菌群分析

合成特异性探针,应用荧光原位杂交（FISH）技术分析阿维菌素废水处理工业化升流式厌氧污泥床（UASB）反应器颗粒污泥产氢产乙酸菌群分布和相对丰度,并测定菌群活性.结果表明：不同形成阶段颗粒污泥表面和内部剖面,产氢产乙酸菌、食丙酸盐产氢产乙酸菌和食丁酸盐产氢产乙酸菌的分布形态相同；产氢产乙酸菌平均相对丰度范围为(10.08±0.81)%～(28.06±2.12)%.成熟期颗粒污泥中产氢产乙酸菌相对丰度最大；颗粒污泥表面产氢产乙酸菌相对丰度大于内部剖面；食丙酸盐产氢产乙酸菌相对丰度大于食丁酸盐产氢产乙酸菌.阿维菌素残留对产氢产乙酸菌群具有抑制作用.不同形成阶段颗粒污泥最大比产乙酸速率范围为0.912～1.145 g/(g·d),且与产氢产乙酸菌群相对丰度变化趋势一致.

Analysis of syntrophic acetogenic bacteria community in anaerobic granular sludge from a full-scale UASB

Probes specific for syntrophic acetogenic bacteria were designed and fluorescence in situ hybridization (FISH) was used to analyze their distribution and relative abundance in anaerobic granular sludge in a full-scale up-flow anaerobic sludge bed (UASB) reactor treating avermectin wastewater, and the activity of syntrophic acetogenic bacteria was also determined. The results indicated that the distribution forms of syntrophic acetogenic bacteria, syntrophic propionate-oxidizing bacteria and syntrophics fatty acid-β-oxidizing syntrophic bacteria were same on the surface and inner face of granular sludge samples in different formation periods. However, the average relative abundances of those syntrophic acetogenic bacteria populations were different, ranging from (10.08±0.81)% to (28.06±2.12)%. The maximum relative abundance of syntrophic acetogenic bacteria was obtained when granular sludge was in mature period. The relative abundances of syntrophic acetogenic bacteria on the inner face were less than those on the surface of granular sludge samples. The relative abundances of syntrophic propionate oxidizing bacteria were larger than those of syntrophics fatty acid-β-oxidizing syntrophic bacteria. The avermectin residue in wastewater showed inhibition effect on syntrophic acetogenic bacteria. The maximum specific acetate rates of granular sludge samples ranged from 0.912 to 1.145 g/(g·d) and their variation tendency was identical with relative abundance of syntrophic acetogenic bacteria．