菊粉对多囊卵巢综合征小鼠外周血、脾脏、肝脏髓源抑制性细胞及血浆炎症因子的影响

目的分析菊粉对多囊卵巢综合征(polycystic ovary syndrome,PCOS)小鼠髓源抑制性细胞(myeloid-derived suppressor cells,MDSCs)、单核细胞样MDSCs(monocytic-MDSCs,M-MDSCs)比例的变化和意义及其与血浆炎症因子的相关关系。方法采用60%高脂饮食联合颈背部皮下注射脱氢表雄酮6 mg/100 g+0.1 ml芝麻油制备PCOS小鼠模型,选取21～27日龄的雌性C57BL/6J小鼠30只,随机分为正常对照组、模型组、菊粉干预组,每组10只。流式细胞仪检测各组小鼠外周血、脾脏、肝脏中MDSCs和M-MDSCs的比例变化以及炎症因子TNF-α、IL-17A和IL-10的水平。结果对MDSCs和M-MDSCs分析发现:与正常对照组小鼠相比,模型组小鼠外周血、脾脏、肝脏中MDSCs和M-MDSCs显著增加,与模型组小鼠相比,菊粉干预组小鼠外周血、脾脏和肝脏MDSCs及M-MDSCs也显著增加。对血浆炎症因子分析发现:TNF-α、IL-17A在模型组与正常对照组小鼠中相比显著增加,而在模型组与菊粉干预组中相比显著降低,IL-10在模型组与正常对照组小鼠中相比显著降低,而在模型组与菊粉干预组中相比增加,但是没有统计学意义。对MDSCs和M-MDSCs与血浆炎症因子TNF-α, IL-17A相关性分析发现:PCOS小鼠外周血、脾脏、肝脏中MDSCs和M-MDSCs与血浆炎症因子TNF-α、IL-17A均呈负相关。结论菊粉能够诱导MDSCs、M-MDSCs在外周血、脾脏和肝脏中募集,减少促炎因子TNF-α、IL-17A的生成,从而延缓疾病的进展。

Effects of inulin on myeloid-derived suppressor cells in peripheral blood,spleen and liver of mice and plasma inflammatory factors in polycystic ovary syndrome

This study was designed to investigate the roles of inulin in the changes of myeloid-derived suppressor cells(MDSCs) and monocytic-MDSCs(M-MDSCs) of polycystic ovary syndrome(PCOS) mice, and the relationship of the changes with plasma inflammatory factors. Total of 30 female C57 BL/6 J mice aged 21-27 days were randomly divided into three groups(10 mice/group): normal control group, model group and inulintreated group. The normal control group was fed a normal diet and injected subcutaneously with 0.1 ml of sesame oil, while the model group and inulin-treated group were fed 60% high-fat diet combinated with subcutaneous injection of dehydroepiandrosterone6 mg/100 g+0.1 ml sesame oil. After successful modeling, the normal control group and the model group were intragastrically administered with normal saline, while the inulin-treated group was treated with inulin. Flow cytometry was used to detect the changes of MDSCs and MMDSCs in peripheral blood, spleen and liver and the levels of plasma inflammatory factors including TNF-α, IL-17 A, IL-10. Data showed that MDSCs and M-MDSCs in the peripheral blood, spleen and liver of the model group were significantly increased compared with the normal control group, while the MDSCs and M-MDSCs in the peripheral blood, spleen and liver of the inulin-treated group were also higher than those in model group mice. The levels of plasma TNF-α, IL-17 A was significantly increased in the model group compared with the normal control group, while significant lower in the model group as compared with the inulin-treated group. IL-10 in the model group was significantly lower than that in the normal control group, while higher as compared with the inulin-treated group with not statistically significant. The number of MDSCs and M-MDSCs in peripheral blood, spleen and liver of PCOS mice increased significantly, which was closely correlated to the occurrence and development of the disease.In conclusion, inulin can induce the recruitment of MDSCs and M-MDSCs in peripheral blood, spleen and liver,reducing the production of pro-inflammatory factors TNF-α and IL-17 A, thereby alleviating the progression of the disease.