Functional modulation of CD8+ T cell by approved novel immune enhancer: Nocardia rubra Cell-Wall Skeletons (Nr-CWS) |
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Affiliation: | 1. Department of Rheumatology and Immunology, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Tiexi District, Shenyang, Liaoning, China;2. Department of Immunology, School of Basic Medical Science, Jinzhou Medical University, No.40, Section 3, Songpo Road, Linghe District, Jinzhou 121000, China;3. Inner Mongolia Research Center of Brucellosis Prevention and Treatment Engineering Technology, Zoonosis Innovation Institute of Inner Mongolia University for Nationalities, Tongliao 028000, China |
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Abstract: | Nocardia rubra cell wall skeleton (Nr-CWS) has been reported to have innate immunostimulating and anti-tumor activities. However, the immunomodulatory effects of Nr-CWS on CD8+ T cells and their related mechanisms are still unknown. In this work, our team purified CD8+T cells from spleen cells and explored the phenotype and function of NR-CWS in vitro on CD8+T cells. We observed that Nr-CWS can significantly up-regulate the expression of CD69 and CD25 on CD8+T cells, with no significant effect on apoptosis or cell death of CD8+T cells that occurs in vitro during culture. In addition, the effect of perforin and granzyme B was increased after Nr-CWS treatment, but did not substantially alter the expression of TRAIL and FasL. A variety of cytokine analyses have shown that of the cytokines examined (IFN-γ, TNF-α, IL-2, IL-4, IL-5, IL-6 and IL-10), only IFN-γ and TNF The increase in -α was more pronounced, and the effect of Nr-CWS in CD8+T cell culture medium on CD8+ T cells was independent of Th cells. Our results demonstrated that Nr-CWS could up-regulate CD69 and CD25 expression on CD8+T cells, promoting IFN-γ and TNF-α secretion, and enhancing perforin and granzyme B production. Thus Nr-CWS may have Immunoaugmenting therapeutic activity via an increase in CD8+T cells response. |
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Keywords: | Nocardia rubra Cell-Wall Skeletons Immune cells Immunoregulation Nr-CWS"} {"#name":"keyword" "$":{"id":"k0030"} "$$":[{"#name":"text" "_":"Nocardia rubra Cell-Wall Skeletons MTS"} {"#name":"keyword" "$":{"id":"k0040"} "$$":[{"#name":"text" "_":"3-(4 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium MACS"} {"#name":"keyword" "$":{"id":"k0050"} "$$":[{"#name":"text" "_":"magnetic activated cell separation FCM"} {"#name":"keyword" "$":{"id":"k0060"} "$$":[{"#name":"text" "_":"flow cytometry IFN-γ"} {"#name":"keyword" "$":{"id":"k0070"} "$$":[{"#name":"text" "_":"interferon-γ FasL"} {"#name":"keyword" "$":{"id":"k0080"} "$$":[{"#name":"text" "_":"Fas ligand TNF-α"} {"#name":"keyword" "$":{"id":"k0090"} "$$":[{"#name":"text" "_":"tumor necrosis factor-α TRAIL"} {"#name":"keyword" "$":{"id":"k0100"} "$$":[{"#name":"text" "_":"TNF-related apoptosis-inducing ligand Prf"} {"#name":"keyword" "$":{"id":"k0110"} "$$":[{"#name":"text" "_":"perforin GrzB"} {"#name":"keyword" "$":{"id":"k0120"} "$$":[{"#name":"text" "_":"granzyme B IL"} {"#name":"keyword" "$":{"id":"k0130"} "$$":[{"#name":"text" "_":"Interleukin |
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